Atas Citologia

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Prof.Fernando Luis Bacelar Lobato

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Células normais escamosas

Tipos diferentes das pilhas squamous - A: pilhas superficial (setas); B: pilhas do ntermediate i; C: pilhas parabasal; D: pilhas metaplastic. (obj. 20x). Normal squamous cells

A: Superficial squamous cells, flat basophilic or eosinophilic cytoplasm. B: Atrophic pattern. (obj. 20X)

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Normal squamous cells

Normal ectocervix: intermediate and superficial squamous cells, basophilic or eosinophilic. Some polymorphs are present. (obj. 10x)


Normal ectocervix: intermediate and superficial squamous cells with basophilic or eosinophilic cytoplasm. (obj. 10x)

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Normal ectocervix: intermediate and superficial squamous cells predominantly basophilic, with a few eosinophilic. (obj. 10x)


Normal ectocervix: basophilic intermediate squamous cells and superficial cells with basophilic or eosinophilic cytoplasm. (obj. 10x)

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Normal ectocervix: normal superficial eosinophilic cells. Some debris in the background. (obj. 20x) Normal squamous cells

Normal ectocervix: intermediate basophilic squamous cells and basophilic and eosinophilic superficial cells (arrows). Note normal nuclear size variations. (obj. 20x).

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Normal ectocervix: rare basophilic intermediate squamous cells and many superficial basophilic and eosinophilic cells. Note the nuclear sizes are different. Two keratinized cells with brownish cytoplasm(arrows). (obj. 20x). Normal squamous cells

Normal ectocervix: basophilic intermediate squamous cells. Some polymorphs. (obj. 20x)

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Normal ectocervix: basophilic squamous cells with histiocytes or macrophages and polymorphs. (obj. 20x) Normal squamous cells

Normal ectocervix: superficial squamous cells with basophilic or eosinophilic cytoplasm. One polymorph (arrow). (obj. 20x)

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Normal ectocervix: intermediate basophilic squamous cells and basophilic and eosinophilic superficial cells. Note the presence of mature squamous metaplasia (arrows). (obj. 20x) Normal squamous cells

Normal ectocervix: basophilic intermediate squamous cells and superficial basophilic and eosinophilic cells. (obj. 20x)

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Normal ectocervix: basophilic intermediate cells, basophilic and eosinophilic superficial squamous cells. (obj. 40x) Normal squamous cells

Normal ectocervix: basophilic intermediate squamous cells. Note some cytoplasms are very pale. (obj. 40x)

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Liquid based preparation: normal smear, intermediate and superficial squamous cells. (obj. 40x) Normal squamous cells

Mid-cycle smear (ovulatory phase): eosinophilic superficial squamous cells, clean background. (obj. 10x)

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Mid-cycle (ovulatory) smear: cervical mucus forming fern-like crystalline structures and eosinophilic superficial squamous cells. (obj. 2.5x) Normal squamous cells

Luteal phase with basophilic squamous cells with folded cytoplasm. (obj. 10x)

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Luteal phase with abundant Lactobacilli and cytolysis (naked nuclei and cytoplasmic debris in the background) and basophilic squamous cells with cytoplasmic folding. (obj. 10x)

Cytopathology of the uterine cervix - digital atlas Frappart L., Fontanière B., Lucas E., Sankaranarayanan R.


Basophilic squamous cells from intermediate layers. Polymorphs are present. (obj. 10x) Cytopathology of the uterine cervix - digital atlas Frappart L., Fontanière B., Lucas E., Sankaranarayanan R.

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Luteal phase with basophilic squamous cells and folded cytoplasm, forming aggregates. (obj. 10x) Normal squamous cells

Luteal phase with marked cytolysis and abundant Doderlein bacilli. Naked nuclei from intermediate cells and cytoplasmic debris. (obj. 40x)

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Squamous metaplasia

A and B: mature squamous metaplasia. C: immature squamous metaplasia. D: immature squamous metaplasia with pattern of transitional metaplasia. (obj. 20x) Mature squamous metaplasia

Intermediate type squamous cells of metaplastic origin. These cells are generally round. (obj. 10x)

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Histiocytes and inflammatory cells

Normal endocervix: histiocytes and rare endocervical glandular cells (arrow). (obj. 10x) Histiocytes and inflammatory cells

Mild inflammation in a normal endocervix: secretory glandular cells (ellipse) with histiocytes (arrow) and polymorphs. (obj. 20x)

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Normal endocervix: mixture of endocervical glandular cells (ellipse) and histiocytes (arrows) or macrophages. It may be difficult to distinguish glandular cells from histiocytes. (obj. 10x) Histiocytes and inflammatory cells

Normal endocervix with mild inflammation: mixture of polymorphs and histiocytes or macrophages. (obj. 20x)

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Normal endocervix: endocervical mucus with naked nuclei from normal cylindrical cells. Some histiocytes (ellipse) and polymorphs are present. (obj. 10x) Cytopathology of the uterine cervix - digital atlas Frappart L., Fontanière B., Lucas E., Sankaranarayanan R. Histiocytes and inflammatory cells

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Normal endocervix: histiocytes with micro-vacuolated cytoplasm. (obj. 40x) Cytopathology of the uterine cervix - digital atlas Frappart L., Fontanière B., Lucas E., Sankaranarayanan R. Histiocytes and inflammatory cells

Atrophic normal endocervix (menopause): columnar cells (ellipse) and histiocytes may be difficult to distinguish. (obj. 40x) Cytopathology of the uterine cervix - digital atlas

Frappart L., Fontanière B., Lucas E., Sankaranarayanan R.

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Atrophic menopausal cervix: many histiocytes are visible in the endocervical sample. (obj. 40x) Histiocytes and inflammatory cells

Normal endocervix: histiocytes and polymorphs, high magnification. (obj. 40x) Cytopathology of the uterine cervix - digital atlas Frappart L., Fontanière B., Lucas E., Sankaranarayanan R.

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Normal cervix: a superficial squamous cell with eosinophilic cytoplasm with polymorphs, histiocytes (black arrow) and macrophages (purple arrows). (obj. 40x) Histiocytes and inflammatory cells

Endocervical smear: high magnification of histiocytes. (obj. 40x)

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Normal but inflammatory endocervical smear: a multinucleated histiocyte among many polymorphs. (obj. 10x)


Normal but inflammatory endocervical smear: a multinucleated histiocyte among many polymorphs. (obj. 20x)

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Immature squamous/transitional metaplasia

Immature squamous metaplasia: a sheet of metaplastic cells, moderately cohesive with their cytoplasmic processes. Some isolated squamous and columnar endocervical cells are present. (obj. 10x)


Immature squamous metaplasia : a sheet of metaplastic cells, moderately cohesive with their cytoplasmic processes. Some isolated squamous and columnar endocervical cells are present. (obj. 20x) Cytopathology of the uterine cervix - digital atlas Frappart L., Fontanière B., Lucas E., Sankaranarayanan R.

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Transition zone: a sheet of immature metaplastic cells (repair cells), nuclei with clear chromatin and visible nucleoli. Inflammatory background. (obj. 20x) Immature squamous/transitional metaplasia

Transition zone (TZ) smear: sheet of metaplastic or repair cells (clear chromatin and visible nucleoli) in a bloody and inflammatory background. (obj. 10x)

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Transition zone: typical squamous metaplastic cells, loosely cohesive (field A) or forming a sheet (field B), surrounded by normal squamous cells, in a bloody and inflammatory background. (obj. 20x) Immature squamous/transitional metaplasia

Transition zone (TZ): sheet of metaplastic or repair cells (clear chromatin and visible nucleoli). Some maturing isolated cells (field B-ellipse). (obj. 40x)

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Transition zone (TZ): sheet of metaplastic or repair cells (clear chromatin and visible nucleoli). Some maturing isolated cells (field A). (obj. 40x) Immature squamous/transitional metaplasia

Transition zone: typical squamous metaplastic cells forming a sheet, in a bloody and inflammatory background. High magnification. (obj. 40x)

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Transition zone: immature metaplastic cells. (obj. 40x) Immature squamous/transitional metaplasia

Transition zone (TZ): sheet of immature metaplastic cells. (obj. 40x).

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Transition zone (TZ): sheet of immature metaplastic cells. (obj. 40x) Immature squamous/transitional metaplasia

Transition zone (TZ): sheet of immature metaplastic cells. (obj. 40x)

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Liquid-based cytology: group of immature squamous metaplastic cells. (obj. 20x)


Liquid-based cytology: group of immature metaplastic cells. (obj. 40x)

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Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Longitudinal nuclear grooves (arrows). (obj. 20x, 2 different fields A and B)


Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Some longitudinal nuclear grooves (arrows) (field B). (field A: obj. 20x, field B: obj. 40x)

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Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Longitudinal nuclear grooves (arrows). (obj. 20x)


Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Some longitudinal nuclear grooves (arrow). (obj. 20x)

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Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Some longitudinal typical nuclear grooves (arrows). Non specific cytoplasmic inclusion. (obj. 40x)


Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Some longitudinal typical nuclear grooves (arrows). (obj. 40x)

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Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Longitudinal nuclear grooves. (obj. 20x)


Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Longitudinal nuclear grooves. (field A: obj. 10x, field B: obj. 20x) Cytopathology of the uterine cervix - digital atlas

Frappart L., Fontanière B., Lucas E., Sankaranarayanan R.

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Transitional metaplasia: immature cells with clear and oval nuclei, with visible nucleoli. Some longitudinal typical nuclear grooves. (obj. 40x)

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