Biologia Genmica
2 Semestre, 2017
Recombinao Homloga e Stio-Especfica
Prof. Marcos Tlio
mtoliveria@fcav.unesp.br
Faculdade de Cincias Agrrias e Veterinrias de Jaboticabal
Instituto de Biocincias, Letras e Cincias Exatas de S.J.R.P.
Universidade Estadual Paulista Jlio de Mesquita Filho
mailto:mtoliveria@fcav.unesp.br
Aula Passada - Reparo de DNA
Recombinao
Recombinao para Reparo
Double-Strand
DNA Breaks
NHEJ
Recombinao
Homloga
Recombinao
Homloga
Recombinao
Homloga
Recombinao Homloga - Reparo
Sistema RecBCD de E. coli
Sistema RecBCD de E. coli
Invaso de ssDNA - RecA de E. coli
Invaso de ssDNA - RecA de E. coli
Yu et al., 2001. PNAS 98:841924
Invaso de ssDNA - RecA de E. coli
Invaso de ssDNA
- RecA de E. coli
Migrao de ssDNA - RuvAB de E. coli
Migrao de ssDNA - RuvAB de E. coli
https://www.sheffield.ac.uk/mbb/ruva/ruva-040
Resoluo - RuvC de E. coli
Recombinao
Meitica
Recombinao
Meitica
Troca de Sexo? S levedura mesmo...
Troca de Sexo? S levedura mesmo...
Recombinao Homloga - Resumo
Rad54, Rad5, BLM, WRN,
RECQ1, RECQ5, FANCM, etc. RecG
Embaralhando o Genoma...
Recombinao Stio-Especfica
Recombinao Stio-
Especfica
Serina e Tirosina Recombinases
Integrase do Bacterifago Lambda
Hin
Recombinase
de Salmonella
XerCD Recombinase de E. coli
XerCD Recombinase de E. coli
XerCD Recombinase de E. coli
Transposio
Transposio
Cut and Paste
Retrotransposons/
Retrovirus
Retrotransposons/Retrovirus
Recombinao/
Reparo e Evoluo
de Genomas
Mecanismos de duplicao gnica
Brown, 2002. Genomes, 2nd ed.
http://www.ncbi.nlm.nih.gov/books/NBK21112/
Brown, 2002. Genomes, 2nd ed.
http://www.ncbi.nlm.nih.gov/books/NBK21112/
Mecanismos de duplicao gnica
8.8 Morphological Complexity Evolves by Adding New Gene Functions
FIGURE 17: Common eukaryotic proteins are
concerned with essential cellular functions
FIGURE 18: Increasing complexity in eukaryotes is accompanied by
accumulation of new proteins for transmembrane and extracellular functions
Famlias de Genes/Protenas
8.10 Globin Clusters Arise by Duplication
and Divergence
FIGURE 20: Each of the alpha-like and beta-like globin gene families is organized
into a single cluster that includes functional genes and pseudogenes (psi)
8.10 Globin Clusters Arise by Duplication and Divergence
FIGURE 21: Different
hemoglobin genes are
expressed during
embryonic, fetal, and
adult periods of
human development
8.10 Globin Clusters Arise by Duplication
and Divergence
8.10 Globin Clusters Arise by Duplication and Divergence
FIGURE 22: Seven mouse genes include two early embryonic genes, one
late embryonic gene, two adult genes, and two pseudogenes
8.11 Pseudogenes Are Nonfunctional
Gene Copies
Some pseudogenes may gain functions different from
those of their parent genes, such as regulation of gene
expression, and take on different names.
FIGURE 23: Many
changes have
occurred in a beta-
globin gene since it
became a
pseudogene
FIGURE 24: Most human RP pseudogenes are of recent origin; many are
shared with the chimpanzee but absent from rodents
Adapted from S. Balasubramanian, et al., Genome Biol. 20 (2009): R2.
Genes Homeobox
Brown, 2002. Genomes, 2nd ed.
http://www.ncbi.nlm.nih.gov/books/NBK21112/
Exon/Domain Shuffling
Brown, 2002. Genomes, 2nd ed.
http://www.ncbi.nlm.nih.gov/books/NBK21112/
Exon Shuffling
Brown, 2002. Genomes, 2nd ed.
http://www.ncbi.nlm.nih.gov/books/NBK21112/
Exon/Domain Shuffling
Brown, 2002. Genomes, 2nd ed.
http://www.ncbi.nlm.nih.gov/books/NBK21112/
Brown, 2002. Genomes, 2nd ed.
http://www.ncbi.nlm.nih.gov/books/NBK2
1112/
Duplicao de genomas inteiros
Genoma de Plantas = Baguna!
http://chibba.agtec.uga.edu/duplication/
FIGURE 13: DNA content of the haploid genome increases with morphological
complexity of lower eukaryotes, but varies among higher eukaryotes
Genoma de Plantas = Baguna!
Hiptese 2R de duplicao do genoma de
vertebrados
Da mitocndria para o ncleo: NUMTs
Bhazkani-Covo et al., 2010. Molecular
Poltergeists: Mitochondrial DNA
Copies (numts) in Sequenced Nuclear
Genomes. PLoS Genetics, e:1000834.
Do cloroplasto para
o ncleo, eu at
entendo, mas do
cloroplasto de uma
alga para o ncleo
de um animal
Rumpho et al., 2011. J Exp Biol, 214:
3003-11.
Evoluo de Blocos de Genes:
Inverses Cromossmicas
Evoluo de Blocos de
Genes:
Inverses Cromossmicas
Evoluo de Blocos de Genes:
Inverses Cromossmicas
Ananina et al., 2004. GMB, 27:61-69.
