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BIOTECNOLOGIA DE BIOTECNOLOGIA DE MICRORGANISMOS MICRORGANISMOS Prof. Dr. Marcos Pileggi Prof. Dr. Marcos Pileggi

BIOTECNOLOGIA DE MICRORGANISMOS

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BIOTECNOLOGIA DE MICRORGANISMOS. Prof. Dr. Marcos Pileggi. Controle Biológico. Compostagem Maltose. Compostagem Lipídios. FASE DE OXIDAÇÃO (BIOESTABILIZAÇÃO). FASE DE MATURAÇÃO (HUMIFICAÇÃO). T E M P E R A T U R A 0 C. 65. 30. termofílica. mesofílica. 20. 30. 4. 75. - PowerPoint PPT Presentation

Text of BIOTECNOLOGIA DE MICRORGANISMOS

  • BIOTECNOLOGIA DE MICRORGANISMOS

    Prof. Dr. Marcos Pileggi

  • Controle Biolgico

  • Compostagem Maltose

  • Compostagem Lipdios

  • Compostagem Maltose

  • Biorremediao

  • Produo de cpsulas Biorremediao de herbicidas

  • Biorremediao Diesel

  • Endofticos Alagados

  • Guignardia isolada de Confrei

  • Endofticos

  • Identificao fungos endofticos

  • Grf1

    64.2054706765134.019640400444.887999262582.5690786353305.029383742149.028948418283.4297422748108.681837164

    8C

    8H

    9C

    9H

    SC

    SH

    ZC

    ZH

    Meios sem/com herbicida

    Ativ/Cat

    Catalase (CAT)

    Plan1

    BactriaX protAbs/R1Abs/R2M(Abs)Ativ/CATBactriaX protAbs/R1Abs/R2M(Abs)Ativ/GR

    8C0.616675-0.036-0.042-0.039-0.00000098980.015416875-64.205470676564.20547067658C1.0610.1290.130.12950.07373581010.053051.3899304451

    8H0.428-0.056-0.057-0.0565-0.0000014340.0107-134.0196404004134.01964040048H0.70660.1330.1490.1410.07966976260.035332.2550173406

    9C0.58804-0.0155-0.0365-0.026-0.00000065990.014701-44.887999262544.88799926259C0.315730.0430.03950.041250.02819917440.01578651.7862841293

    9h0.79306-0.072-0.057-0.0645-0.00000163710.0198265-82.569078635382.56907863539H0.65020.0310.0310.0310.02291021670.032510.7047129104

    SC0.728065-0.1415-0.296-0.21875-0.0000055520.018201625-305.029383742305.029383742Z0.568630.0220.0160.0190.01671826630.02843150.5880191426

    SH0.89241-0.133-0.129-0.131-0.00000332490.02231025-149.0289484182149.0289484182ZH0.36570.0160.0160.0160.01517027860.0182850.8296570215

    Z0.568885-0.0335-0.06-0.04675-0.00000118650.014222125-83.429742274883.4297422748

    ZH0.9785-0.0545-0.155-0.10475-0.00000265860.0244625-108.681837164108.681837164

    BactriasAtiv/CAT

    8C64.2054706765,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,

    8H134.0196404004

    9C44.8879992625

    9H82.5690786353

    SC305.029383742

    SH149.0289484182

    ZC83.4297422748

    ZH108.681837164

    BactriasAtiv/GR

    8C1.3899304451

    8H2.2550173406

    9C1.7862841293

    9H0.7047129104

    Z0.5880191426

    ZH0.8296570215

    GST

    X protBactriaAbs 1Ativ/GST

    1.0618C0.084262.584247.4872761546

    0.70668H0.0398124.4148176.0752901217

    0.315739C0.381187.883762.328571881

    0.65029H0.3821194.1321836.561058136

    0.56863Z000

    0.3657ZH000

    BactriasAtiv/GST

    8C247.4872761546

    8H176.0752901217

    9C3762.328571881

    9H1836.561058136

    Z0

    ZH0

    Plan1

    00000000

    8C

    8H

    9C

    9H

    SC

    SH

    ZC

    ZH

    Meios com/sem herbicida

    Ativ/Cat

    Catalase (CAT)

    Plan2

    000000

    8C

    8H

    9C

    9H

    Z

    ZH

    Meios com/sem herbicida

    Ativ/GR

    Glutationa Redutase (GR)

    Plan3

    000000

    8C

    8H

    9C

    9H

    Z

    ZH

    Meio s/com herbicida

    Ativ/GST

    GST

    TempoControle0,1mM0,2mM

    0h14.492736718714.4863214.75963

    3h19.514216656324.09481238835.0307178147

    6h21.933372300718.84648321728.7701797194

    12h22.461307829523.09625084799.1230668245

    24h23.07676601928.428736753414.5151195762

    36h16.099590847110.789899677712.9682764706

    48h7.55414616669.187950036814.9653742515

    72h6.093387290518.516460953225.1318199555

    96h9.78651486995.933863523718.4108975804

    TempoControle0,1mM0,2mM

    0h0.79137534460.7913650.796325

    3h0.49305085430.89865806232.8705376223

    6h0.43375238130.61130939931.6621458555

    12h0.69725983331.67958844051.9209924933

    24h0.5032885570.72920062043.5466669241

    36h0.85044630170.57975342843.290658332

    48h0.63923179190.64016085630.5824206833

    72h0.30181675150.63054377740.5705450492

    96h0.31365800950.60771948951.5658129561

    000

    000

    000

    000

    000

    000

    000

    000

    000

    Controle

    0,1mM

    0,2mM

    Tempo(h)

    Ativ/GR

    GR

    REP 1REP2DP - CAT-21.33-22-0.021665

    -21.33-220.47376154340h-27.5-26.6-0.02705

    -27.5-270.35355339063h-27.5-27-0.02725

    -27.5-270.35355339066h-29-29-0.029

    -29-29012h-42.5-43-0.04275

    -42.5-430.353553390624h-18-18.5-0.01825

    -18-18.50.353553390636h-7-7.5-0.00725

    -7-7.50.353553390648h-3-2.5-0.00275

    -3-2.50.353553390672h-4-4.5-0.00425

    -4-4.50.353553390696h

    -18-19-0.0185

    -18-190.7071067812-9-9-0.009

    -9-90-4-5-0.0045

    -4-50.7071067812-5.5-6.5-0.006

    -5.5-6.50.7071067812-6-6-0.006

    -6-60-2-1.25-0.001625

    -2-1.250.5303300859-3-4-0.0035

    -3-40.7071067812-1-1.66-0.00133

    -1-1.660.4666904756

    -2-2.5-0.00225

    -2-2.50.3535533906-9.2-9.5-0.00935

    -9.2-9.50.2121320344-6-5-0.0055

    -6-50.7071067812-5.5-6-0.00575

    -5.5-60.3535533906-7-5-0.006

    -4.75-50.1767766953-3.5-3.5-0.0035

    -3.5-3.50-6.5-5.5-0.006

    -6.5-5.50.7071067812-4.5-4.5-0.0045

    -4.5-4.50

  • Polimorfismo Biorremediao herbicidas

  • AbordagensCaracterizao de produtosEspectrofotometriaCromatografiaIdentificaoRNA ribossomalGenomamRNA

  • EstoquesBactriasGlicerol 50% -20CDMSO 50% -20CFungosPlugs de miclio e esporos em gua 4CMiclio em papel de filtro 20CCatalogao