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FNI 2C EM 1 Transmission Electron Microscope

FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

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Page 1: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 1

Transmission Electron Microscope

Page 2: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 2

TEM Image of E. Coli

Page 3: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 3

TEM Image of Mitochondria

Page 4: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 4

TEM Image of Computer Chip

Page 5: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 5

SEM Image compared to TEM Image

Page 6: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 6

Transmission Electron Microscope

http://www.matter.org.uk/tem/ http://em-outreach.ucsd.edu/web-course/toc.html http://www.amazon.com/Transmission-Electron-Microscopy-

Textbook-Materials/dp/038776500X/ref=sr_1_1?ie=UTF8&s=books&qid=1224191760&sr=8-1

Page 7: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 7

TEM Operation

Page 8: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 8

UW Eau Claire’s TEM

Page 9: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 9

UW Eau Claire’s TEM

Page 10: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 10

TEM Phosphor Screen

Page 11: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 11

TEM Control Panel

Page 12: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 12

TEM Grids

All TEM’s ever done have examined only .3 mm3 of material

Page 13: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 13

TEM Components

Page 14: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 14

TEM Sample Preparation and Theory of Operation Samples for TEM must be electron transparent. That means they

must be thin enough for electrons to pass through. It is very difficult to achieve this. After the sample has been polished very thin an ion mill is used to

perform the final stages. An ion mill uses an argon plasma to remove material from the

sample. High energy argon ions strike the sample and atoms of the sample

are knocked away. TEMs can be used to image atoms. Some scientists have combined TEM chambers with reactors so

they can watch chemical reactions in progress.

Page 15: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 15

FIB Sample Preparation for TEM

Page 16: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 16

Electron Microscope Vendors

JEOL http://www.jeol.com/ Hitachi http://www.hitachi-hitec-uk.com/ Zeiss www.smt.zeiss.com/nts FEI www.feicompany.com Camscan www.camscan-usa.com Elektronen-Optik www.eos-do.com Tescan www.tescan.com KLA/Tencor/Amray http://www.kla-tencor.com/

Page 17: FNI 2C EM 1 Transmission Electron Microscope. FNI 2C EM2 TEM Image of E. Coli

FNI 2C EM 17

Notes

Growth of carbon nanotubes inside environmental TEM.