ic-2200 um

High Technology, Inc. Immunochem 2200 User Manual

Page 1 of 10 Rev. A

HTI Immunochem-2200 Incubator Shaker

Revision Log The following is a list of major changes and additions that have been made to this manual since it was first released. See the accompanying Release Notes for specific changes to the software and hardware between manual updates.

Rev Date Description A 12/17/12 User Manual Released


Page 2 of 10 Rev. 12.17.2012

Contents

1. Safety Precautions

2. General Information

3. Getting Started

4. Operation

5. Specifications

6. Technical Maintenance

7. Warranty, Reclamation Information

8. CE Marking


Page 3 of 10 Rev. 12.17.2012

1. Safety precautions The following symbols mean:

Caution: Read these operating instructions fully before use and pay particular attention to sections containing this symbol

Caution: Surfaces can become hot during use.

Use only as specified by the operating instructions or the intrinsic protection

may be impaired.

The unit must be stored and transported only in a horizontal position (see

marking on the package).

. Connect only to a power supply with a voltage corresponding to that on the serial number label.

. Do not use other power supply units than supplied with the unit.

. Ensure that the mains switch and isolating device (power supply connector) are easily accessible during use.

Connect only to a power supply which provides a safety earth (ground) terminal.

Do not operate the unit outside the laboratory premises.

Do not operate the unit in premises with aggressive or explosive chemical

mixtures.

Before moving, disconnect at the power supply socket.

For model 2200-4: Do not close the lid if the microplate fixation screws are not turned in to avoid damage.

If liquid is spilt inside the unit, disconnect it from the power supply and have it

checked by a competent person.

Before using any cleaning or decontamination method except those

recommended by the manufacturer, user should check with the manufacturer

that the proposed method will not damage the equipment.

. The unit should be protected from shocks and falling.


Page 4 of 10 Rev. 12.17.2012

2. General Information IMMUNOCHEM 2200 / IMMUNOCHEM 2200-4 is designed for shaking 1-4 standard 96-well

microplates in the thermostating mode. It was designed using the multi-system principle, which

allows using it as three independent devices:

1) Incubator for lasting incubation without shaking of micro quantities (insect, plant

cell cultures, etc.) in immunoplates;

2) Microplate Shaker for operation in the cold room or other conditions, which do not require

temperature stabilisation;

3) microplate instrument for immunochemistry and molecular diagnostics,

where the requirements to the result reproducibility and thus to the precise method

regulation are particularly high.

IMMUNOCHEM 2200/ IMMUNOCHEM 2200-4 provides:

-Gentle or vigorous shaking of samples;

-Regulation, stabilization and indication of rotation speed;

-Even shaking amplitude throughout Instrument platform;

-Setting and indication of the required operation time;

-Automatic stop of platform movement after the set time expires,

-Indication of the current operation time,

-Setting and indication of the required temperature on the platform.

The device can be used in:

-cytochemistry for in situ reactions;

-immunochemistry for immunofermentative reactions;

-biochemistry for enzyme and protein analysis;

-molecular chemistry for matrix analysis.

Instrument does not have a switch to turn it off and it can run in the platform heating mode, i.e.

thermostat mode as long as needed.

The time of the continuous work in the Shaker mode is restricted by the continuous work of the

motor, guaranteed by the manufacturer (i.e. 3500 hr of continuous work).

The maximum guaranteed number of diagnostic cycles in the Instrument mode, which require

15-30 min work in one cycle, is 7000-14000 times.

External 12V power supply unit is used to power the device. It makes it safe for work in the

cold room, where condensation may cause leakage current from the mains.


Page 5 of 10 Rev. 12.17.2012

3. Getting started 3.1. Unpacking.

Remove packing materials carefully and retain for future shipment or storage of the unit.

3.2. Complete set.

Instrument set includes:

-Immunochem 2200 .................................................... 1 pce

-Spare rubber belt ...................................................... 2 pce

-External power supply unit ........................................ 1 pce

-Operating Manual; Specifications; Certificate ......... 1 copy

3.3.Place instrument upon even horizontal surface.

3.4.Plug the external power supply unit (AC/DC adapter supplied with the shaker) into the 12 V

socket at the rear side of the Instrument.


Page 6 of 10 Rev. 12.17.2012

4. Operation of Instrument 4.1. Connect power supply unit to the mains and switch ON the power switch located

on the rear panel of the unit.

4.2. The display will turn on with the upper line (set point) showing time, RPM and

temperature set at the factory (15 min, 1000 RPM, 37.0 C) and the lower line

(actual point) showing current readings of the same parameters (STOP - time, 000

- RPM, thermoblock temperature C, which automatically starts rising according to

the temperature set in the upper line). The time of temperature stabilisation

depends on the initial temperature, but does not exceed 15-20 min, if the set

temperature is 37.0 C.

How to set the necessary parameters. Use the readings in the upper line of the display (set point), while setting the necessary

parameters.

Reaction time (TIME)

4.3. With the help of and keys (Fig. 1/1) set the required working time interval in hours and minutes (increment - 1 min). If the key is pressed for

longer time the increment becomes bigger.

Shaking intensity (RPM)

4.4. With the help of and keys (Fig. 1/2) set the required shaking intensity in revolutions per minute (increment 10 RPM). If the key is pressed for longer

time the increment becomes bigger.

Reaction temperature (T, C)

4.5. With the help of and keys (Fig. 1/3) set the necessary temperature (increment 0.1C). If the key is pressed for longer time the increment becomes

bigger.

Attention: It is possible to turn off heating of the thermoblock only by setting the required temperature below 25 C (the display will show OFF - T,C - set point). In this

mode Instrument can be used in the cold rooms as a mixing device without

thermoregulation.


Page 7 of 10 Rev. 12.17.2012

Program execution

After the thermal stabilisation of the Instrument (when the set and current temperature

readings become the same):

4.6. Microplate fixation:

IMMUNOCHEM 2200-2: Place microplates on the platform and fix it with the special holder

by pressing it against the plate covers.

IMMUNOCHEM 2200-4: Unscrew the fixation screws. Place microplates on the platform and

fix them by turning in the fixation screws.

Caution! The microplate fixation screws must always be turned in to avoid damage. Turn in the fixation screws completely when microplates are removed from or placed on the

platform.

4.7. Press the RPM-RUN/STOP key (Fig. 1/4). The platform will start rotation and the timer indicator will start counting up the time interval (with 1 min precision).

4.8. At the end of the program the platform motion stops and the timer shows the flashing

reading STOP accompanied by the repetitive sound signal until the RPM-RUN/STOP key is pressed.

4.9. If the working time is not set (or deleted) and the timer indicator in the upper line shows

00:00, pressing the RPM-RUN/STOP key cause continuous work of the Instrument until the RPM-RUN/STOP key is pressed again.

Please note, that the platform temperature will be constantly maintained in accordance with

the set temperature that allows using the device again without pre-heating.

4.10. If required, there is possibility to restart the timer when it is running. Press the TIME-RUN/STOP key once (Fig. 1/5) to stop the timer. Press the TIME-RUN/STOP key again to restart the timer.

4.11. The platform motion can be stopped at any time by pressing the RPM-RUN/STOP key. In this case the program realisation and the platform motion stops and timer is set back to zero

switching into the STOP mode.


Page 8 of 10 Rev. 12.17.2012

At the end of the set time period the platform movement is stopped automatically,but the heating

have to be stopped by reducing the temperature with the T,C key (Fig. 1/3 lower button) till the OFF sign appears in the upper part of the

display.

4.12. At the end of operation turn OFF the unit with power switch at the rear panel and

disconnect the external power supply unit from the mains.

5. Specifications 5.1.Temperature regulation range ........................ +25C ... +60 C

(Instrument provides stable thermoregulation when the set temperature is at least 5C higher

than the ambient temperature)

5.2.Nominal regulation accuracy ................................... 0.1C

5.3.Temperature uniformity over the platform ................... 0.2C

5.4.Orbit ........................................................................... 2 mm

5.5.Max. height of microtest plate ................................ 18 mm

Unit for higher plates can be produced on request.

5.6.Speed regulation ................... 250-1200 RPM (increment 10 RPM)

5.7.Independent timer with sound signal 0 - 96 hrs (increment 1 min)

5.8.Time of thermoblock heating from RT till 37C ...... 15-20 min

5.9.Display .......................................................... 16x2 signs, LCD

5.10. ..............................................................Power supply External power supply

Input AC 100-240 V 50/60Hz, output DC 12V 5.11.Current/power consumption

5.12.Number of microplates

5.13.Platform dimensions

5.14.Dimensions

5.15.Weight, incl. power supply

5.16.The unit is designed for operation in closed laboratory rooms at ambient temperature from

+5C to +40C and maximum relative humidity 80% for temperatures up to 31C decreasing

linearly to 50% relative humidity at 40C.

To improve the design manufacturer reserves the right to make changes in specification

without prior notice.

IMMUNOCHEM 2200-2 IMMUNOCHEM 2200-4 12V DC, 3,3 A / 40W 12V DC, 4,15 A / 50W 2 4 250 x 150 mm 210 x 290 mm 270x260x125 mm 380x390x140 mm Up to 7 kg Up to 9 kg


Page 9 of 10 Rev. 12.17.2012

6. Technical Maintenance 6.1. If service is required, switch off the unit and contact High Technology, Inc. or your local

High Technology, Inc. representative for repairs.

6.2. It is allowed to perform the maintenance and all types of repair operations only to the

authorised specialists or service-engineers who have done a special training at the

manufacturing company.

6.3. Instrument design allows easy replacement of any failed part with a new one. The following

spare parts can be ordered for replacement: Main processor board, Power supply board,

Front control board, Speed control board, Motor, Cables, Rubber belt, LCD display, External

power supply unit, Platform with thermoblock, Main body with the heating lid.

6.4. Cleaning liquids that do not contain

concentrate organic solvents, alkali or acid

can be used for device cleaning.

6.5. Standard ethanol (75%) can be used for

disinfection.

6.6. Rubber belt replacement (fig.2). Remove 4

fixation screws on the shaker bottom and

remove the bottom plate. Replace the rubber

belt. Assemble the unit.

Fig.2


Page 10 of 10 Rev. 12.17.2012

7. Warranty. Reclamation information 7.1. The Manufacturer warrants the compliance of the device with the requirements of

Specifications provided the consumer follows the operation, storage and transportation

requirements.

7.2.The warranted service life of the device from the date of delivery to the customer is 12 months.

7.3.If any manufacturing defects are discovered by the customer, an unsatisfactory -equipment

report shall be compiled, certified and sent to the manufacturer at your local dealer (distributor)

address.

7.4.For your records complete.

The following information will be required in the event that warranty service comes necessary.

Complete and retain for your records.

Model IMMUNOCHEM 2200 / IMMUNOCHEM 2200-4

Serial number _______________________

Date of sale _______________________

Documents

ic-2200 um