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0 2,000 4,000 6,0000

1,000

2,000

3,000

4,000

5,000

P2X7

Membrane Proteome Array

No

rma

lize

d T

arg

et

Bin

din

g

�ŶƚĂŐŽŶŝƐƚ�D�ďƐ �ŐŽŶŝƐƚ�D�ď

-11 -10 -9 -80

20

40

60

80

100

MAb 3

MAb 1MAb 2

Log [MAb] (M)

No

rma

lize

d C

a2+ F

lux

(%)

0 60 120 1800

20406080

100120140

20 nM

5 nM

1.25 nM

0 nM

[MAb]

MAbadded

Time (sec)

Ca

2+ I

nfl

ux

(% O

ver

Bas

elin

e)