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Preparo de biblioteca para sequenciamento Exemplos dos ... · PDF file DNA Insert Rd2 SP Index Rdl SP Index Rd2 SP' E. Denature and amplify for final product Library construction begins

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  • Introdução à Bioinformática – IQB001

    Rafael Dias Mesquita [email protected]

    Laboratório de Bioinformática

    Departamento de Bioquímica

    Preparo de biblioteca para sequenciamento Exemplos dos kits TruSeq e Nextera da Illumina

    mailto:[email protected]

  • Preliminar: PCR

    http://bioinfo.iq.ufrj.br/graduacao/links/08%20PCR.swf

    http://bioinfo.iq.ufrj.br/graduacao/links/08%20PCR.swf

  • Kit TruSeq Illumina

  • DNA double strands created based on FW and RV strands

  • Strand specificity

    U U U U U U This strand is NOT used as template on PCR

    DNA double strand created based only on FW strand

  • Strand specificity

    U U U U U U This strand is NOT used as template on PCR

    DNA double strand created based only on FW strand

    https://support.illumina.com/content/dam/illumina-support/documents/documentation/ chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq- stranded-total-rna-workflow-reference-1000000040499-00.pdf

    https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf

  • Kit Nextera Illumina

  • https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/nextera-dna-flex.html

    Kit Nextera Illumina

    https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/nextera-dna-flex.html

  • https://www.youtube.com/watch?v=-kTcFZxP6kM

    Resumo de vários kits de preparação da amostra

    https://www.youtube.com/watch?v=-kTcFZxP6kM

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