OS ovos de resitenca uma viagem a lo largo do tempo e o espaco Iglesias cbl 2013 palestra

Os ovos de resistencia: uma viagem através do tempo e espaco?

Carlos Iglesias, Claudia Bonecker, Jorge Coronel, Cristina Crispim, Eneida Eskinazi, Paulina Maia-Barbosa, Eliana Panarelli, Jorge Portinho, Jayme Santangelo, Edinaldo Santos-Silva & Evelyn Zoppi

Special session: Comunidades dormentes

[email protected]

O estado da arte de sistemas (sub)tropical da America do Sul

SUMMARY:

Work overview

Brief introduction

Who are we?

Our working questions

What we did?

Some results

Future research

How to continue?


Os ovos de resistencia, uma viagem através do tempo e espaco? O estado da arte de sistemas (sub)tropical da America do Sul

Work overview

• Bibliographic search (Scholar.google, EBSCO)

• Identification of “local” researchers + @sedimentos

• Invitation to participate by filling question form

• Information on researchers location, year started

working, thesis, approaches, publications

• What we already know.....

•Our future work, together?



Brief Introduction



Brief introduction

DORMANCY: Suspension of vital functions for a certain time to

overcome harsh environmental conditions. 2 Types: Quiescence

and Diapause

Widespread in several phyla from Bacteria to Vertebrates.

Different protective structures:

• Gemmules

• Resistant eggs

• Cocoons

• Cysts

• Statoblasts

• Ephippia



Freshwater zooplankton

Brief introduction

Harsh environment conditions



SEASONAL: Phtoperiod and Temerature (desedation int emporal systems) BIOTIC: Predation and food availability

Brief introduction

Formation of egg bank during subsequent seasons



Brief introduction

Egg bank functioning



• Size

• Vertical and

horizontal

structure

• Phenology

• Signals?

• Amount?

Can be cosidered the archive/Overlooking it may lead to erroneous interpretations!!

Mortality by: •Predation

• Infection

•Senescence

Who are we?

•Evelyn Zoppi U. Central de Venezuela

•Edinaldo Nelson INPA, Amazonas

•Cristina Crispim U.F. De Paraíba

•Jorge Coronel U.Mayor de San Simón, Bolivia

•Paulina Maia-Barbosa, U.F. Minas Gerais

•Eliana Panarelli, U.E. Minas Gerais

•Eneida Eskinazi, U.F. Ouro Preto

•Claudia Bonecker UE Maringá, Paraná

•Jayme Santangelo, U.F. Rural do Rio de Janeiro

•Jorge Portinho, U.E. Paulista

•Carlos Iglesias, CURE, Udelar, Uruguay



Who are we?




•Jorge CoronelU.Mayor de San Simón, Bolivia










1984 2013

2002

1998

2003

2002

2001

2008

2005

2005

2011

2009

Who are we?



Edinaldo Nelson

Evelyn Zoppi Cristina

Crispim

Eneida Eskinazi

Jorge Coronel

Eliana Panarelli

Paulina Maia-

Barbosa

Claudia Bonecker

Jayme Santangelo

Carlos Iglesias

Jorge Portinho

Reinaldo Bozelli

Araujo

Lourdes El-moor

Loureiro

Silvia MC Casanova





•Jorge CoronelU.Mayor de San Simón, Bolivia










Permanent Temporal Marine

Permanent Temporal

Permanent Temporal

Permanent

Temporal

Permanent Temporal

Temporal

Permanent Temporal

Permanent Temporal

Permanent

Permanent Temporal


• FIELD WORKS/Description of the egg banks principally in

temporary systems, Increasing in the last years also permanent

systems (Shallow lakes) and coastal lagoons

• Determination of best methodologies (ex situ) to succeed with

eclosion of individuals from sediments.

• Separation of eggs Identification (sugar flotation method)

•Generation of keys

• Experimental approaches to determine which factors (cues)

influence eclosion (temeperature, light, predators)



What we did?

• Our scientific production measured in papers and pos graduate thesis

• 6 MSc Thesis, 6 PhD (including ours Eliana, Jayme, Carlos, Jorge, Jorge)

• 18 PAPERS, 11 in Regional Journals, 7 in International Jurnals

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0 2 4 6 8 10

brasil

uruguay

venezuela

bolivia regional internacional

What we did?




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1 2 3 4 5 6 7 8 9 10 11 12 13

Publications per year

regional internacional

What we did?




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1 2 3 4 5 6 7 8 9 10 11 12 13



What we did?




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Some results • Uruguay, Iglesias et al. 2011

9 of 18 lakes Daphnia ephippia

6 of them only found in S samples

3 of them only found in water


in situ

Large sized cladocerans appeared after fish exclusion

When fish was added then they disappeared or their size was diminished

Some results

Igleisias et al., 2011 Hydrobiologia 667:133-147


Some results • Bolivia, Jorge S. Coronel et al (61 ponds)

17 Cladoceran species in W and S

7 species only in S

5 only in water samples


Some results • Brasil, Maia-Barbosa et al 2003

•Spatial heterogeinity of

egg bank in egg density

and composition


Some results • Brasil,


Some results • Brasil,

Future research

EXPERiMENTAL

Lab microcosm Mesocosms “Whole lake”

Empirical evidence

Plants Fish Zooplankton

Empirical-experimental approach

Temperature

Why and when hatch?

PREDATION By fish &/or invertebrates Jeppesen et al 2007, Meerhoff 2008 Lacerot 2010

PHYSIOLOGICAL Growth, reprod, respiration rates Moore et al 1996

FOOD QUANTITY Food threshold higher for

large species in warmer lakes Duncan, 1989

FOOD QUALITY Cyanobacteria and inedible algae Elser et al 2000 De Mott et 2001

Light

Why and when rest?

Latitudinal analyses of ephippia occurence and sediment incubation

in samples. Thinking in some collaboration toghether

Future research

Study the “potential” zooplankton community vs anual varitation in the water colum

Future research

Investigate composition & abundance Calculate saturation & rarefaction

Also invetigate correlation between Active and latent zooplankton

Future research

Emergence and ephippia

production phenology

Future research

http://images.google.com.uy/imgres?imgurl=evodevo.uoregon.edu/images/daphnia.gif&imgrefurl=http://evodevo.uoregon.edu/people/Omilian.html&h=399&w=300&prev=/images?q=Daphnia&svnum=10&hl=es&lr=&ie=UTF-8






Future research

Emergence and ephippia

production phenology

Future research

Plastic Tissue Culture Flask

Tygon Tubing

Friction fit: mouth of flask fits snuggly over tubing

Tubing attached to funnel and funnel to bottle with “hot glue”

Small plastic funnel

Plastic bottle from water cooler (with bottom cut off)

Mesh windows attached with hot glue

Sheet-metal collar, with hole for bottle: rests on sediment surface

Metal tubing legs (we made them with electrical conduit) They project about 20 cm into sediment to hold trap in place

Photo by CM Kearns Draw by Nelson G. Hairston

Doubts!

How to induce eclosion in lake samples

To dry or not to dry

Cool or not, how long

Culture meddium vs lake water

Hipoclorite

Emergence Traps frequency of sampling, how many of them, where

Ephippia production traps…………..

Who are we?



Edinaldo Nelson

Evelyn Zoppi Cristina

Crispim

Eneida Eskinazi

Jorge Coronel

Eliana Panarelli

Paulina Maia-

Barbosa

Claudia Bonecker

Jayme Santangelo

Carlos Iglesias

Jorge Portinho

Reinaldo Bozelli

Araujo

Lourdes El-moor

Loureiro

Silvia MC Casanova

Thanks!!

Thanks for finantial support to PDT, ANII, CSIC –Udelar Uruguay

Gracias!!

Obrigado!!

Education

OS ovos de resitenca uma viagem a lo largo do tempo e o espaco Iglesias cbl 2013 palestra