FAPESP :: Fundação de Amparo à Pesquisa do Estado de ......2 Universidade da Região da Campanha, RS, Brasil. 3 Universidad Nacional de La Plata, Argentina. S.E.Freire SOUTH AMERICAN

CLASSIFICATION AND PHYLOGENY OF SOUTH AMERICAN

GNAPHALIEAE (ASTERACEAE)

FROM MORPHOLOGY AND MOLECULAR DATA

Susana E. Freire

Instituto Darwinion, San Isidro, Argentina.

• Achyrocline (Less.) DC., 32 spp.

• Anaphalis DC., c. 110 spp.

• Antennaria J. Gaertn., c. 40 spp.

• Belloa J. Rémy, 1 or 12 spp

• Berroa Beauverd, 1 sp

• Chevreulia Cass, 5 spp

• Chionolaena DC.,17 spp (incl. Gnaphaliothamnus

Kirp., Leucopholis Gardn.)

• Cuatrecasasiella H. Rob. 2 spp.(= Luciliopsis Wedd.)

• Facelis Cass., 3 spp.

• Gamochaeta Wedd., c. 60 spp

• Gamochaetopsis Anderb. & S.E. Freire, 1 sp

• Jalcophila Dillon & Sagástegui,

• Loricaria Wedd., 19 spp

• Lucilia Cass. (incl. Oligandra Less.), 8 or 12 spp

• Luciliocline Anderb. & S.E. Freire, 5 or 13-16 spp

• Raouliopsis S.F. Blake , 2 spp

• Micropsis DC., c. 5 spp

• Mniodes (A. Gray) Benth., 5 spp.

• Pseudognaphalium Kirp., c. 90 spp.

• Psilocarphus Nutt., 8 spp.

• Stenophalium Anderb. 3 spp

• Stuckertiella Beauverd, 2 spp.

22 genera

15

exclusively

from South

America

V Southamerican Gnaphalieae

S.E.Freire SOUTH AMERICAN GNAPHALIEAE (ASTERACEAE) 5/12/2011

PHYLOGENY OF GAMOCHAETA

PHYLOGENY OF “LUCILIA GROUP”


• To test the monophyly of “Lucilia Group”

• To test the monophyly of each genus of “Lucilia Group”

• To test the monophyly of Gamochaeta and to

establish the relationships among its species

USING MOLECULAR AND MORPHOLOGICAL

EVIDENCE:

PHYLOGENY OF “LUCILIA GROUP” (GNAPHALIEAE, ASTERACEAE)

INFERRED FROM MORPHOLOGY AND MOLECULAR DATA

M. A. Chemisquy1, S. E. Freire1, S. G. Beck 2 y E. Urtubey1

1 Instituto Darwinion, Argentina. 2 Universidad Mayor de San Andrés, Bolivia


• Belloa J. Rémy, 12 especies


• Chevreulia Cass, 5 spp

• Chionolaena DC.,17 spp

• Facelis Cass., 3 spp

• Gnaphaliothamnus Kirp., 1 sp

• Leucopholis Gardn., 4 spp

• Loricaria Wedd. 19 spp

• Lucilia Cass., 8 spp.

• Luciliopsis Wedd., 3 spp

• Mniodes (A. Gray) Benth., 5 spp

• Oligandra Less., 1 spp

• Raouliopsis S.F. Blake , 2 spp

• Jalcophila Dillon & Sagástegui, 2 spp

• Novenia S.E. Freire, 1 sp

13 géneros

V “Lucilia Group” Merxmüller, Leins & Roessler 1977

1986


LORICARIINAE

RELHANIINAE

CASSINIINAE

ANGIANTHIINAE

GNAPHALIINAE

SUBTRIBES OF GNAPHALIEAE Anderberg, 1991


• Belloa J. Rémy, 12 spp


• Chevreulia Cass., 5 spp

• Cuatrecasasiella H. Rob., 2 spp

• Facelis Cass., 3 spp

• Lucilia Cass., 11 spp.


“Lucilia Group” sensu Anderberg, 1991

7

géneros Anderberg,

1991


• Belloa J. Rémy

• Berroa Beauverd

• Chevreulia Cass

• Cuatrecasasiella H. Rob. 1985 (= Luciliopsis Wedd. )

• Facelis Cass.

• Lucilia Cass.

• Jalcophila Dillon & Sagástegui

• Novenia S.E. Freire

• Chionolaena DC.

• Gnaphaliothamnus Kirp.

• Raouliopsis S.F. Blake

• Mniodes (A. Gray) Benth.

• Loricaria Wedd.

• Leucopholis Gardn. (= Chionolaeana DC.) – Freire 1993

• Oligandra Less. (= Lucilia Cass.) – Freire 1989

“

CASSINIINAE

LORICARIINAE

ASTEREAE

V “Lucilia Group” Merxmüller et al. 1977/Anderberg 1991


• Belloa J. Rémy

• Berroa Beauverd

• Chevreulia Cass.

• Cuatrecasasiella H. Rob.

• Facelis Cass.

• Lucilia Cass.


• Gamochaetopsis Anderberg & S.E. Freire, 1 sp

• Luciliocline Anderberg & S.E. Freire, 5 spp

9

géneros Anderberg &

Freire, 1991 3

“Lucilia Group” sensu Anderberg, 1991/Anderberg & Freire, 1991

7

géneros Anderberg,

1991


SYNAPOMORPHIES OF “LUCILIA GROUP”

GNAPHALIINAE - “Lucilia Group”, Anderberg & Freire 1991


• Style branches dorsally

pubescent

• Pappus bristles connate

• Florets purple

• Belloa de 9 1 sp

• Berroa 1 sp

• Chevreulia 5 spp

• Cuatrecasasiella 2 spp

• Facelis 3 spp

• Lucilia de 8 12 spp

• Jalcophila de 3 2 spp

• Gamochaetopsis 1 sp

• Luciliocline de 8 12 spp

“Lucilia Group” sensu Dillon, 2000, 2003


METHODS

4 molecular markers: trnL-F and matK

(chloroplast), ITS and ETS (from the nucleus)

• Molecular matrices were aligned using the online

application MAFFT

• Phylogenetic analyses were performed using the

software TNT

• Branch support was evaluated using jackknife expressed

as absolute values and as frequency differences (GC)

• Gaps were treated as missing data

2 Phylogenetic analyses:

Molecular matrix composed of 4 markers

Molecular matrix + morphological matrix (36 caracteres)


Belloa (4)

Berroa (1)

Chevreulia (1)

Cuatrecasasiella (1)

Facelis (3)

Gamochaetopsis (1)

Jalcophila (1)

Lucilia (4)

Luciliocline (3)

GNAPHALINEAS: Achyrocline, Edmondia, Gamochaeta, Gnaphalium

Helichrysum, Lasiopogon, Leontopodium, Logfia, Pseudognaphalium,

Stuckertiella, Stuartina, Vellereophyton,

LORICARINEAS: Mniodes, Loricaria

CASSININEAS: Acanthocladium, Antennaria, Anaphalis,

Anaxeton, Cassinia, Ewartia, Langebergia

RHELANINEAS: Oedera, Relhania

ASTEREAS: Novenia, Solidago, Chiliotrichium, Chiliophyllum,

Baccharis, Aster

In group

Out group

TAXA


Combined trnL-F,matK, ITS and ETS

Tribe Astereae

Tribe Gnaphalieae

Lucilia Group


Novenia, 1 sp - Argentina, Bolivia, Peru


Tribe Gnaphalieae

Lucilia Group


CHEVREULIA , 5 spp - Argentina, Bolivia, Brasil,

Chile, Paraguay, Uruguay, Falkland Islands

CUATRECASASIELLA, 2 spp

Ecuador to Argentina


Tribe Gnaphalieae

Lucilia Group


JALCOPHILA, 3 spp - Ecuador, Peru, Bolivia

Jalcophila. boliviensis


Tribe Gnaphalieae

Lucilia Group


LORICARIA, 19 spp - Colombia to Bolivia

Tribe Gnaphalieae

Lucilia Group


LUCILIOCLINE , 5 spp - Peru, Bolivia, Argentina


BELLOA, 9 spp – Venezuela to Chile

Luciliocline subspicata

Belloa schultzii

Tribe Gnaphalieae

Lucilia Group



BELLOA, 9 spp – Venezuela to Chile

MNIODES, 4 spp – Peru, Bolivia

Tribe Gnaphalieae

Lucilia Group



GAMOCHAETOPSIS 1sp - Argentina, Chile

GAMOCHAETA

c. 60 spp-New World

STUCKERTIELLA, 1 sp - Argentina, Bolivia, Peru

Tribe Gnaphalieae

Lucilia Group


Belloa chilensis– Argentina, Chile


FACELIS, 3 spp - Argentina, Bolivia,

Brazil, Chile, Paraguay, Peru, Uruguay


Tribe Gnaphalieae

Lucilia Group


LUCILIA 8 spp -Argentina, Bolivia, Brasil,

Chile, Paraguay, Uruguay

BERROA 1 sp Argentina, Uruguay

Combined Molecular and Morphological Evidence

Tribu Astereae

Tribu Gnaphalieae

Lucilia Group


Combined Molecular and Morphological Evidence

Tribu Gnaphalieae

Lucilia Group


PHYLOGENY OF GAMOCHAETA (GNAPHALIEAE, ASTERACEAE)

INFERRED FROM MORPHOLOGY AND MOLECULAR DATA

E. Urtubey1, S. E. Freire1, A. López1, M. A. Chemisquy1, L. Paz Deble 2 & N. Bayón3

1 Instituto Darwinion, Argentina. 2 Universidade da Região da Campanha, RS, Brasil. 3 Universidad

Nacional de La Plata, Argentina.


METHODS

2 molecular markers: ITS and ETS (from the

nucleus)

• Molecular matrices were aligned using the online

application MAFFT

• Phylogenetic analyses were performed using the

software TNT

• Branch support was evaluated using jackknife expressed

as absolute values and as frequency differences (GC)

• Gaps were treated as missing data

Phylogenetic analysis:


Taxon sampling: 27 species of Gamochaeta

Belloa chilensis – Argentina, Chile


Combined ETS-ITS

Combined ETS-ITS/ ETS

ETS-ITS

ETS

STUCKERTIELLA, 1 sp

Argentina, Bolivia, Peru



ETS-ITS

ETS

GAMOCHAETOPSIS ,1 sp Argentina - Chile



GAMOCHAETOPSIS ,1 sp Argentina - Chile


Gamochaeta nivalis - Argentina, Chile

Combined ETS-ITS

Gamochaeta filaginea, Argentina-Brazil-

Uruguay


G. axillaris

G. filaginea

G. nigrevestis

G. platensis

G. subfalcata

Combined ETS-ITS

Gamochaeta purpurea

América del Norte

Gamochaeta neuquensis

Chile-Argentina


Combined ETS-ITS

Gamochaeta longepedicellata

Argentina, Bolivia

Gamochaeta lulioana - Bolivia


Gamochaeta stachydifolia - Argentina, Brazil


Combined ETS-ITS

Gamochaeta grazielae - Brazil

1. “Lucilia group” sensu Anderb. is paraphyletic.

CONCLUSIONS – Lucilia Group

2. Molecular + Morphological evidence confirm the monophyly of 8 of 9

genera of “Lucilia group” sensu Anderb.:

Berroa, Chevreulia, Cuatrecasasiella, Facelis, Gamochaetopsis,

Jalcophila, Lucilia, Luciliocline sensu Anderb. & Freire.


3. Molecular analysis:

Mniodes (Loricariinae) forms a monophyletic group with Belloa

sensu Anderb. & Freire (excl. Type) and Luciliocline sensu Anderb. &

Freire.

4. Molecular + Morphological characters:

Jalcophila boliviensis belongs to genus Jalcophila.

5. Molecular and Molecular + Morphological confirm:

Belloa as a monotypic genus.

Jalcophila

Cuatrecasasiella

Chevreulia *

Luciliocline

“Belloas”

Mniodes

Belloa

Berroa

Facelis *

6. Molecular Evidence: Two clades


Loricaria

Gamochaeta

* Stucke rtiella*

Gamochaetopsis

Lucilia *

PHYLOGENY OF GAMOCHAETA

PHYLOGENY OF “LUCILIA GROUP”


• To add Micropsis (Gnaphaliinae) and Chionolaeana

(Cassiniinae)

• To add species from Chile

• To combine molecular and morphological evidence

WORK IN PROGRESS:

AGRADECIMIENTOS

• •

ACKNOWLEDGMENTS

• Finnacial support was provided by Consejo Nacional

de InvestigacionesCientíficas y Técnicas (CONICET)

Argentina- PIP 112-200801-02196.

• To C. Ezcurra, (UNCOMA) and F. Zuloaga et al. (SI), for

specimens of Belloa chilensis, and Belloa piptolepis,

respectively.

• To J. M. Bonifacino (UDeLaR) for his photograph of

Chevreulia sarmentosa.


Documents

FAPESP :: Fundação de Amparo à Pesquisa do Estado de ......2 Universidade da Região da Campanha, RS, Brasil. 3 Universidad Nacional de La Plata, Argentina. S.E.Freire SOUTH AMERICAN