INCT de Processos Redox

em Biomedicina

http://www2.iq.usp.br/redoxoma/

Pesquisas

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Principais Tópicos de Pesquisa O estudo dos processos redox tem alto potencial para elucidar mecanismos biológicos

integradores, devido à prevalência desses processos e de seus fortes efeitos. Muitos estudos envolvem o

papel dos mecanismos redox em doenças. Contudo, a complexidade deste campo tem aumentado com o

conhecimento das estruturas biológicas, das relações estrutura-função, da patologia molecular, da

genômica, da proteômica e da metabolômica do estresse oxidativo. Assim, uma abordagem

multidisciplinar se tornou essencial para avanços significativos no campo. Nosso objetivo é realizar

estudos que permitam a concepção e avaliação de estratégias antioxidantes com aplicabilidade clínica.

Abordamos questões relevantes para superar as limitações no conhecimento dos processos redox, como:

1) Formação de ROS e controle em sistemas biológicos; 2) A reatividade química de espécies reativas de

oxigênio (ROS) em ambientes biológicos e consequentes mudanças na estrutura e função de

biomoléculas; 3) Mecanismos e redes envolvidas em processos de sinalização redox relevantes para

doenças humanas; 4) Diagnóstico e aplicações terapêuticas de processos redox.

Resumo dos Dados Obtidos e Perspectivas

O núcleo da rede Redoxoma começou no Programa Institutos do Milênio e adquiriu experiência

para fazer importantes contribuições para o avanço do conhecimento sobre as bases moleculares dos

processos redox no primeiro ano do INCT. É impossível resumir os resultados obtidos em poucas linhas,

mas aqueles que abrem novas perspectivas foram destaque em nosso Relatório Anual (ver Relatório

Anual em http://www2.iq.usp.br/redoxoma/). Com relação aos biomarcadores, foi desenvolvido um método

fluorescente, altamente sensível, para a detecção de aldeídos de colesterol formado por ozônio e oxigênio

singlete (Mansano et al., Anal Chem. 82:6775-81, 2010). Em relação às enzimas antioxidantes, novos

insights sobre a especificidade das interações tiorredoxina-tiorredoxina redutase foram fornecidos (Oliveira

et al., Biochemistry 49:3317-26, 2010). Demonstrou-se que a geração de espécies reativas de oxigênio

pelas mitocôndrias é substrato-, tecido- e sítio-específica (Tahara et al., Free Radic Biol Med 46:1283-97,

2010). Com relação aos danos a biomoléculas, foi demonstrado que a proteína de recombinação RAD52

coopera com a proteína OGG1 para reparar lesões oxidativas no DNA de células de mamíferos (de Souza-

Pinto et al., Mol Cell Biol 29:4441-54, 2009). A elucidação de mecanismos pelos quais a dissulfeto

isomerase protéica (PDI) regula processos redox via interação com NADPH oxidase vem revelando vias

de sinalização importantes e passíveis de intervenção terapêutica em vários modelos de doença

cardiovascular (Santos et al., Antioxid Redox Signal. 11:2409-27, 2009). Também foi definida uma rede de

proteínas em Saccharomyces cerevisiae relacionada ao processo de envelhecimento (Barea e Bonatto,

Mech Ag Devel 13:444-60, 2009). Aplicações terapêuticas de processos redox foram exploradas com o

antioxidante não clássico tempol, que se mostrou eficaz no tratamento da esclerose múltipla em

camundongos (Tsuhako et al., Free Radic Biol Med 48:704-12, 2010).

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Publicações

Comentários gerais

Os grupos que compõem a rede Redoxoma produziram 194 artigos científicos, 14 capítulos de

livros e editaram um livro, no período de 2009-2010. Algumas das nossas contribuições científicas foram

publicadas em conceituadas revistas gerais, tais como FASEB J, J Biol Chem, J Mol Biol e Biochemistry;

outras contribuições foram publicadas em revistas especializadas de alto impacto, tais como Ant Redox

Sig e Free Rad Biol Med. A maioria dessas contribuições é consequência da qualidade dos vários grupos

que compõem o Redoxoma. A influência da rede em si é ainda recente, mas a interação entre os vários

grupos está se expandindo, como atestam os vários trabalhos publicados em conjunto (Fig. 1). A lista

completa das publicações de 2009 e 2010 é apresentada abaixo, com as publicações em revistas de

impacto ≥ 5 em azul. Na área de Bioquímica e Biologia Molecular, 18,7% dos periódicos têm esse índice

de impacto, de acordo com o Journal Citation Reports ISI Web of Knowledge (HTTP://admin-

apps.isiknowledge.com/JCR/JCR).

Figura 1- Interactoma dos pesquisadores do INCT-Redoxoma. As linhas cheias indicam interações que já resultaram em publicações conjuntas e as linhas pontilhadas indicam interações em progresso.

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Lista de publicações

Periódicos

• Aamann MD, Sorensen MM, Hvitby C, Berquist BR, Muftuoglu M, Tian J, de Souza-Pinto NC, Scheibye-Knudsen M, Wilson Iii DM, Stevnsner T, Bohr VA. Cockayne syndrome group B protein promotes mitochondrial DNA stability by supporting the DNA repair association with the mitochondrial membrane. FASEB J. 2010; 24:2334-46.

• Alberici LC, Oliveira HC, Paim BA, Mantello CC, Augusto AC, Zecchin KG, Gurgueira SA, Kowaltowski AJ, Vercesi AE. Mitochondrial ATP-sensitive K(+) channels as redox signals to liver mitochondria in response to hypertriglyceridemia. Free Radic Biol Med. 2009; 47:1432-9

• Alcântara JM, Yamaguchi KL, Veiga-Junior VF, Lima ES. Composição química de óleos essenciais de espécies de Aniba e Licaria e suas atividades antioxidante e antiagregante plaquetária. | Essential oils composition from Aniba and Licaria species and their antioxidant and antiplatelet activities. Quim Nova. 2010; 33:141-145.

• Alves CM, Silva DA, Azzolini AE, Marzocchi-Machado CM, Carvalho JV, Pajuaba AC, Lucisano-Valim YM, Chammas R, Liu FT, Roque-Barreira MC, Mineo JR. Galectin-3 plays a modulatory role in the life span and activation of murine neutrophils during early Toxoplasma gondii infection. Immunobiology. 2010; 215:475-85.

• Alves WA, Matos IO, Takahashi PM, Bastos EL, Martinho H, Ferreira JG, Silva CC, Santos RHdeA, Paduan-Filho A, Ferreira AMdaC. A Chloro-Bridged Linear Chain Imine-Copper(II) Complex and Its Application as an Enzyme-Free Amperometric Biosensor for Hydrogen Peroxide. Eur J Inorg Chem. 2009; 2009:2219-2228.

• Amaral JF, Gomes-Santos AC, Paula-Silva J, Nicoli JR, Vieira LQ, Faria AM, Menezes JS. Antigenic dietary protein guides maturation of the host immune system promoting resistance to Leishmania major infection in C57BL/6 mice. Immunology. 2010; 129:455-64.

• Amarante-Mendes GP, Bortoluci KR, Bozza PT, Chammas R, Viola JP. Paradise revealed: first-class science rocked by the sound of the waves. Cell Death Differ. 2010; 17:1368-72.

• Aveles PR, Criminácio CR, Gonçalves S, Bignelli AT, Claro LM, Siqueira SS, Nakao LS, Pecoits-Filho R. Association between Biomarkers of Carbonyl Stress with Increased Systemic Inflammatory Response in Different Stages of Chronic Kidney Disease and after Renal Transplantation. Nephron Clin Pract. 2010; 116:c294-c299.

• Avila Júnior S, Possamai FP, Budni P, Backes P, Parisotto EB, Rizelio VM, Torres MA, Colepicolo P, Wilhelm Filho D. Occupational airborne contamination in south Brazil: 1. Oxidative stress detected in the blood of coal miners. Ecotoxicology. 2009; 18:1150-7.

• Azzellini MAA, Abbott MP, Machado A, Miranda MTM, Garcia LC, Caramori GF, Gonçalves MB, Petrilli HM, Ferreira AMC. Interactions of di-imine copper(II) complexes with albumin: competitive equilibria, promoted oxidative damage and DFT studies. J Braz Chem Soc. 2010 ;21:1303-1317.

• Barbeiro HV, Barbeiro DF, Debbas V, Souza HP, Laurindo FR, Velasco IT, Soriano FG. Purine nucleotides reduce superoxide production by nitric oxide synthase in a murine sepsis model. Braz J Med Biol Res. 2009; 42:1050-7.

• Barbosa JS, Cabral TM, Ferreira DN, Agnez-Lima LF, de Medeiros SR. Genotoxicity assessment in aquatic environment impacted by the presence of heavy metals.Ecotoxicol Environ Saf. 2010; 73:320-5.

• Barbosa LF, Cerqueira FM, Macedo AF, Garcia CC, Angeli JP, Schumacher RI, Sogayar MC, Augusto O, Carrì MT, Di Mascio P, Medeiros MH. Increased SOD1 association with chromatin, DNA damage, p53 activation, and apoptosis in a cellular model of SOD1-linked ALS. Biochim Biophys Acta. 2010; 1802:462-471.

• Barbosa LF, Garcia CC, Di Mascio P, de Medeiros MH. DNA oxidation, strand-breaks and etheno-adducts formation promoted by Cu, Zn-superoxide dismutase-H2O2 in the presence and absence of bicarbonate. Dalton Trans. 2009; 1450-9.

• Barbosa LF, Garcia CCM, Di Mascio P, Medeiros MHG. DNA oxidation, strand-breaks and etheno-adducts formation promoted by Cu, Zn-superoxide dismutase-H2O2 in the presence and absence of bicarbonate. Dalton Trans. 2009; 1450-1459.

• Barea F, Bonatto D. Aging defined by a chronologic-replicative protein network in Saccharomyces cerevisiae: an interactome analysis. Mech Ageing Dev. 2009; 130:444-60.

• Barros MH, da Cunha FM, Oliveira GA, Tahara EB, Kowaltowski AJ. Yeast as a model to study mitochondrial mechanisms in ageing. Mech Ageing Dev. 2010; 131:494-502.

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• Berra CMB, Menck CFM, Martinez GR, Oliveira CS, Baptista MS, Di Mascio P. Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO(2) and photoactivated methylene blue. Quim Nova. 2010; 33:279-283.

• Borges CB, Bernardes ES, Latorraca EF, Becker AP, Neder L, Chammas R, Roque-Barreira MC, Machado HR, de Oliveira RS. Galectin-3 expression: a useful tool in the differential diagnosis of posterior fossa tumors in children. Childs Nerv Syst. 2010. [Epub ahead of print].

• Bourguignon SC, Castro HC, Santos DO, Alves CR, Ferreira VF, Gama IL, Silva FC, Seguins WS, Pinho RT. Trypanosoma cruzi: in vitro activity of Epoxy-alpha-Lap, a derivative of alpha-lapachone, on trypomastigote and amastigote forms. Exp Parasitol. 2009; 122:91-6.

• Bourguignon SC, Cavalcanti DF, de Souza AM, Castro HC, Rodrigues CR, Albuquerque MG, Santos DO, da Silva GG, da Silva FC, Ferreira VF, de Pinho RT, Alves CR. Trypanosoma cruzi: Insights into naphthoquinone effects on growth and proteinase activity. Exp Parasitol. 2010. [Epub ahead of print].

• Busso C, Tahara EB, Ogusucu R, Augusto O, Ferreira-Junior JR, Tzagoloff A, Kowaltowski AJ, Barros MH. Saccharomyces cerevisiae coq10 null mutants are responsive to antimycin A. FEBS J. 2010; 277:4530-4538.

• Bustos-Valenzuela JC, Halcsik E, Bassi EJ, Demasi MA, Granjeiro JM, Sogayar MC. Expression, purification, bioactivity, and partial characterization of a recombinant human bone morphogenetic protein-7 produced in human 293T cells. Mol Biotechnol. 2010; 46:118-26.

• Cadet J, Douki T, Ravanat JL, Di Mascio P. Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation. Photochem Photobiol Sci. 2009; 8:903-11.

• Campos VR, Abreu PA, Castro HC, Rodrigues CR, Jordão AK, Ferreira VF, de Souza MC, Santos Fda C, Moura LA, Domingos TS, Carvalho C, Sanchez EF, Fuly AL, Cunha AC. Synthesis, biological, and theoretical evaluations of new 1,2,3-triazoles against the hemolytic profile of the Lachesis muta snake venom. Bioorg Med Chem. 2009; 17:7429-34.

• Campos VR, Abreu PA, Castro HC, Rodrigues CR, Jordão AK, Ferreira VF, de Souza MC, Santos Fda C, Moura LA, Domingos TS, Carvalho C, Sanchez EF, Fuly AL, Cunha AC. Synthesis, biological, and theoretical evaluations of new 1,2,3-triazoles against the hemolytic profile of the Lachesis muta snake venom. Bioorg Med Chem. 2009; 17:7429-34.

• Cangussú SD, Souza CC, Campos CF, Vieira LQ, Afonso LC, Arantes RM. Histopathology of Leishmania major infection: revisiting L. major histopathology in the ear dermis infection model. Mem Inst Oswaldo Cruz. 2009; 104:918-22.

• Canugovi C, Maynard S, Bayne AC, Sykora P, Tian J, de Souza-Pinto NC, Croteau DL, Bohr VA. The mitochondrial transcription factor A functions in mitochondrial base excision repair. DNA Repair (Amst). 2010; 9:1080-9.

• Cardoso AR, Cabral-Costa JV, Kowaltowski AJ. Effects of a high fat diet on liver mitochondria: increased ATP-sensitive K+ channel activity and reactive oxygen species generation. J Bioenerg Biomembr. 2010; 42:245-53.

• Cardozo KHM, Vessecchi R, Galembeck SE, Guaratini T, Gates PJ, Pinto E, Lopes NP, Colepicolo P. A Fragmentation study of di-acidic mycosporine-like amino acids in electrospray and nanospray mass spectrometry. J Braz Chem Soc. 2009; 20:1625-1631.

• Carignan MO, Cardozo KH, Oliveira-Silva D, Colepicolo P, Carreto JI. Palythine-threonine, a major novel mycosporine-like amino acid (MAA) isolated from the hermatypic coral Pocillopora capitata. J Photochem Photobiol B. 2009; 94:191-200.

• Carneiro CD, Amorim JC, Cadena SM, Noleto GR, Di Mascio P, Rocha ME, Martinez GR. Effect of flavonoids on 2'-deoxyguanosine and DNA oxidation caused by singlet molecular oxygen. Food Chem Toxicol. 2010; 48:2380-7.

• Carneiro MLB, Porfírio EP, Otake AH, Chammas R, Báo SN, Guillo LA. Morphological alterations and G0/G1 cell cycle arrest induced by curcumin in human SK-MEL-37 melanoma cells. Braz Arch Biol Technol. 2010; 53:343-52.

• Carranza JC, Kowaltowski AJ, Mendonça MA, de Oliveira TC, Gadelha FR, Zingales B. Mitochondrial bioenergetics and redox state are unaltered in Trypanosoma cruzi isolates with compromised mitochondrial complex I subunit genes. J Bioenerg Biomembr. 2009; 41:299-308.

• Carrillo-Sepúlveda MA, Ceravolo GS, Fortes ZB, Carvalho MH, Tostes RC, Laurindo FR, Webb RC, Barreto-Chaves ML. Thyroid hormone stimulates NO production via activation of the PI3K/Akt pathway in vascular myocytes. Cardiovasc Res. 2010; 85:560-70.

• Carvalho MJde, Pedrosa TdoN, Guilhon-Simplicio F, Nunez CV, Ohana DT, Pereira MdeM, Lima ES. Estudo farmacognóstico e atividade in vitro sobre a coagulação sanguínea e agregação plaquetária das folhas de Passiflora nitida Kunth (Passifloraceae). Acta Amazônica. 2010; 40:199-206.

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• Cavalheiro RA, Marin RM, Rocco SA, Cerqueira FM, da Silva CC, Rittner R, Kowaltowski AJ, Vercesi AE, Franchini KG, Castilho RF. Potent cardioprotective effect of the 4-anilinoquinazoline derivative PD153035: involvement of mitochondrial K(ATP) channel activation. PLoS One. 2010; 5:e10666.

• Celotto AC, Fukada SY, Laurindo FR, Haddad R, Eberlin MN, de Oliveira AM. Chronic hyperhomocysteinemia impairs vascular function in ovariectomized rat carotid arteries. Amino Acids. 2010; 38:1515-22.

• Cerqueira FM, Kowaltowski AJ. Commonly adopted caloric restriction protocols often involve malnutrition. Ageing Res Rev. 2010; 9:424-30.

• Chudzinski-Tavassi AM, De-Sá-Júnior PL, Simons SM, Maria DA, de Souza Ventura J, de Fátima Correia Batista I, Faria F, Durães E, Reis EM, Demasi M. A new tick Kunitz type inhibitor, Amblyomin-X, induces tumor cell death by modulating genes related to the cell cycle and targeting the ubiquitin-proteasome system. Toxicon. 2010. [Epub ahead of print].

• Costa MD, Paludo KS, Klassen G, Lopes MH, Mercadante AF, Martins VR, Camargo AA, Nakao LS, Zanata SM. Characterization of a specific interaction between ADAM23 and cellular prion protein. Neurosci Lett. 2009; 461:16-20.

• Costa MJ, Galembeck E, Hermes-Lima M, Beltramini LM, Torres BB. Biochemistry and molecular biology education in Latin America and Iberia. Biochem Mol Biol Educ. 2009; 37:267.

• Croteau DL, de Souza-Pinto NC, Harboe C, Keijzers G, Zhang Y, Becker K, Sheng S, Bohr VA. DNA Repair and the Accumulation of Oxidatively Damaged DNA Are Affected by Fruit Intake in Mice. J Gerontol A Biol Sci Med Sci. 2010. [Epub ahead of print].

• Cunha AC, Gomes AT, Ferreira VF, de Souza MC, Neves MG, Tomé AC, Silva AM, Cavaleiro JA. Synthesis of beta-arylporphyrinsand oligophenylenediporphyrins by the Suzuki-Miyaura reaction. Synthesis (Stuttgart). 2010; 2010:510-14.

• Cunha VRR, Ferreira AMdaC, Constantino VRL, Tronto J, Valim JB. Hidróxidos duplos lamelares: nanopartículas inorgânicas para armazenamento e liberação de espécies de interesse biológico e terapêutico. | Layered double hydroxides: inorganic nanoparticles for storage and release of species of biological and therapeutic interest. Quim Nova. 2010; 33:159-171.

• Cussiol JR, Alegria TG, Szweda LI, Netto LE. Ohr (organic hydroperoxide resistance protein) possesses a previously undescribed activity: Lipoyl-dependent peroxidase. J Biol Chem. 2010; 285:21943-50.

• da Silva EN Jr, de Deus CF, Cavalcanti BC, Pessoa C, Costa-Lotufo LV, Montenegro RC, de Moraes MO, Pinto Mdo C, de Simone CA, Ferreira VF, Goulart MO, Andrade CK, Pinto AV. 3-arylamino and 3-alkoxy-nor-beta-lapachone derivatives: synthesis and cytotoxicity against cancer cell lines. J Med Chem. 2010; 53:504-8.

• da Silveira VC, Caramori GF, Abbott MP, Gonçalves MB, Petrilli HM, da Costa Ferreira AM. Oxindole-Schiff base copper(II) complexes interactions with human serum albumin: spectroscopic, oxidative damage, and computational studies. J Inorg Biochem. 2009; 103:1331-41.

• Damy SB, Camargo RS, Chammas R, Figueiredo LF. Fundamental aspects on animal research as applied to experimental surgery. Rev Assoc Med Bras. 2010; 56:103-11.

• Dani C, Oliboni LS, Vanderlinde R, Pra D, Dias JF, Yoneama ML, Bonatto D, Salvador M, Henriques JA. Antioxidant activity and phenolic and mineral content of rose grape juice. J Med Food. 2009; 12:188-92.

• de Andrade CR, de Campos GA, Tirapelli CR, Laurindo FR, Haddad R, Eberlin MN, de Oliveira AM. Adult rats are more sensitive to the vascular effects induced by hyperhomocysteinemia than young rats. Vascul Pharmacol. 2010; 53:99-106.

• de Andrade CR, Leite PF, Montezano AC, Casolari DA, Yogi A, Tostes RC, Haddad R, Eberlin MN, Laurindo FR, de Souza HP, Corrêa FM, de Oliveira AM. Increased endothelin-1 reactivity and endothelial dysfunction in carotid arteries from rats with hyperhomocysteinemia. Br J Pharmacol. 2009; 157:568-80.

• de Brito LC, da Rosa MA, Lopes VS, e Ferreira EF, Vieira LQ, Sobrinho AP. Brazilian HIV-infected population: assessment of the needs of endodontic treatment in the post-highly active antiretroviral therapy era. J Endod. 2009; 35:1178-81.

• de Oliveira Demarchi AC, Zambuzzi WF, Paiva KB, da Silva-Valenzuela MG, Nunes FD, de Cássia Sávio Figueira R, Sasahara RM, Demasi MA, Winnischofer SM, Sogayar MC, Granjeiro JM. Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9. Cell Tissue Res. 2010; 340:61-9.

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• de Oliveira IM, Zanotto-Filho A, Moreira JC, Bonatto D, Henriques JA. The role of two putative nitroreductases, Frm2p and Hbn1p, in the oxidative stress response in Saccharomyces cerevisiae. Yeast. 2010; 27:89-102.

• de Oliveira JT, de Matos AJ, Gomes J, Vilanova M, Hespanhol V, Manninen A, Rutteman G, Chammas R, de Fátima Gärtner M, Soares Bernardes E. Coordinated expression of galectin-3 and galectin-3-binding sites in malignant mammary tumours: implications for tumour metastasis. Glycobiology. 2010; 20:1341-1352.

• de Oliveira Mendes ST, de Brito LC, Rezende TM, de Oliveira Reis R, Cardoso FP, Vieira LQ, Ribeiro Sobrinho AP. A decrease in the innate immune response to infection in the presence of root canal sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010; 109:315-23.

• de Oliveira SI, Andrade LN, Onuchic AC, Nonogaki S, Fernandes PD, Pinheiro MC, Rohde CB, Chammas R, Jancar S. Platelet-activating factor receptor (PAF-R)-dependent pathways control tumour growth and tumour response to chemotherapy. BMC Cancer. 2010; 10:200.

• de Souza Malaspina TS, Zambuzzi WF, dos Santos CX, Campanelli AP, Laurindo FR, Sogayar MC, Granjeiro JM. A possible mechanism of low molecular weight protein tyrosine phosphatase (LMW-PTP) activity modulation by glutathione action during human osteoblast differentiation. Arch Oral Biol. 2009; 54:642-50.

• de Souza-Pinto NC, Aamann MD, Kulikowicz T, Stevnsner TV, Bohr VA. Mitochondrial helicases and mitochondrial genome maintenance. Mech Ageing Dev. 2010; 131:503-10.

• de Souza-Pinto NC, Mason PA, Hashiguchi K, Weissman L, Tian J, Guay D, Lebel M, Stevnsner TV, Rasmussen LJ, Bohr VA. Novel DNA mismatch-repair activity involving YB-1 in human mitochondria. DNA Repair (Amst). 2009; 8:704-19.

• de Souza-Pinto NC, Maynard S, Hashiguchi K, Hu J, Muftuoglu M, Bohr VA. The recombination protein RAD52 cooperates with the excision repair protein OGG1 for the repair of oxidative lesions in mammalian cells. Mol Cell Biol. 2009; 29:4441-54.

• Degaki TL, Demasi MA, Sogayar MC. Overexpression of Nrp/b (nuclear restrict protein in brain) suppresses the malignant phenotype in the C6/ST1 glioma cell line. J Steroid Biochem Mol Biol. 2009; 117:107-16.

• Demasi M, Felicio AL, Pacheco AO, Leite HG, Lima C, Andrade LH. Studies on terrein as a new class of proteasome inhibitors. J Braz Chem Soc. 2010; 21:299-305.

• Dos Santos LM, Santos MM, de Souza Silva HP, Arantes RM, Nicoli JR, Vieira LQ. Monoassociation with probiotic Lactobacillus delbrueckii UFV-H2b20 stimulates the immune system and protects germfree mice against Listeria monocytogenes infection. Med Microbiol Immunol. 2010. [Epub ahead of print].

• Egito LC, Dos Santos PE, do Amaral VS, Batistuzzo de Medeiros SR, Agnez-Lima LF. Use of native species Crenicichla menezesi (Ariidae) as a model for in situ evaluation of genotoxicity in surface water. Sci Total Environ. 2010; 402:6042-46.

• Elias N, Tarasoutchi F, Spina GS, Sampaio RO, Pomerantzeff PM, Laurindo FR, Grinberg M. Myocardial fibrosis and ventricular remodeling in severe chronic aortic regurgitation. Arq Bras Cardiol.2009;92:63-7.

• Fernandes DC, Manoel AH, Wosniak J Jr, Laurindo FR. Protein disulfide isomerase overexpression in vascular smooth muscle cells induces spontaneous preemptive NADPH oxidase activation and Nox1 mRNA expression: effects of nitrosothiol exposure. Arch Biochem Biophys. 2009; 484:197-204.

• Ferreira CL, Marques FL, Okamoto MR, Otake AH, Sugai Y, Mikata Y, Storr T, Bowen M, Yano S, Adam MJ, Chammas R, Orvig C. Cationic technetium and rhenium complexes with pendant carbohydrates. Appl Radiat Isot. 2010; 68:1087-93.

• Ferreira Mde L, Vasconcelos TR, de Carvalho EM, Lourenço MC, Wardell SM, Wardell JL, Ferreira VF, de Souza MV. Synthesis and antitubercular activity of novel Schiff bases derived from d-mannitol. Carbohydr Res.2009; 344:2042-7.

• Ferreira SB, de Carvalho da Silva F, Bezerra FA, Lourenço MC, Kaiser CR, Pinto AC, Ferreira VF. Synthesis of alpha- and beta-pyran naphthoquinones as a new class of antitubercular agents. Arch Pharm (Weinheim). 2010; 343:81-90.

• Ferreira SB, Sodero AC, Cardoso MF, Lima ES, Kaiser CR, Silva FP, Ferreira VF. Synthesis, biological activity, and molecular modeling studies of 1H-1,2,3-triazole derivatives of carbohydrates as alpha-glucosidases inhibitors. J Med Chem. 2010; 53:2364-75.

• Ferreira VF, da Rocha DR, Silva FCda. Potencialidades e oportunidades na química da sacarose e outros açúcares | Potentiality and opportunity in the chemistry of sucrose and other sugars. Quim Nova. 2009; 32:623-638.

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• Ferreira VF, Ferreira SB, de Carvalho da Silva F. Strategies for the synthesis of bioactive pyran naphthoquinones. Org Biomol Chem. 2010. [Epub ahead of print].

• Ferreira-Cravo M, Welker AF, Hermes-Lima M. The connection between oxidative stress and estivation in gastropods and anurans. Prog Mol Subcell Biol. 2010; 49:47-61.

• Filomeni G, Piccirillo S, Graziani I, Cardaci S, Da Costa Ferreira AM, Rotilio G, Ciriolo MR. The isatin-Schiff base copper(II) complex Cu(isaepy)2 acts as delocalized lipophilic cation, yields widespread mitochondrial oxidative damage and induces AMP-activated protein kinase-dependent apoptosis. Carcinogenesis. 2009; 30:1115-24.

• Francisco AI, Vargas MD, Fragoso TP, Carneiro JWM, Casellato A, Silva FC, Ferreira VF, Barbosa JP, Pessoa C, Costa-Lotufo LV, Marinho Filho JDB, Moraes MO, Mangrich AS. Theoretical studies of the tautomerism in 3-(2-R-Phenylhydrazono)-naphthalene- 1,2,4-triones: synthesis of copper(II) complexes and studies of antibacterial and antitumor activities. J Braz Chem Soc. 2010; 21:1293-1302.

• Francisco G, Menezes PR, Eluf-Neto J, Chammas R. Arg72Pro TP53 polymorphism and cancer susceptibility: A comprehensive meta-analysis of 302 case-control studies. Int J Cancer. 2010. [Epub ahead of print].

• Franco CR, Trindade ES, Rocha HA, da Silveira RB, Paludo KS, Chammas R, Veiga SS, Nader HB, Dietrich CP. Glycosaminoglycan chains from alpha5beta1 integrin are involved in fibronectin-dependent cell migration. Biochem Cell Biol. 2009; 87:677-86.

• Franco Lambert AP, Fraga Zandonai A, Bonatto D, Cantarelli Machado D, Pêgas Henriques JA. Differentiation of human adipose-derived adult stem cells into neuronal tissue: does it work? Differentiation. 2009; 77:221-8.

• Freire CPVF, Ferreira SB, Oliveira NSMO, Matsuura ABJ, Gama IL, Silva FC, Souza MCBV, Lima ES, Ferreira VF. Synthesis and biological evaluation of substituted [alpha]- and [beta]-2,3-dihydrofuran naphthoquinones as potent anticandidal agents. Med Chem Commun. 2010; 1:229-232.

• Fujita A, Sato JR, Demasi MA, Sogayar MC, Ferreira CE, Miyano S. Comparing Pearson, Spearman and Hoeffding's D measure for gene expression association analysis. J Bioinform Comput Biol. 2009; 7:663-84.

• Fujita A, Sato JR, Demasi MA, Yamaguchi R, Shimamura T, Ferreira CE, Sogayar MC, Miyano S. Inferring Contagion in Regulatory Networks. IEEE/ACM Trans Comput Biol Bioinform. 2010. [Epub ahead of print].

• Galembeck E, Hermes-Lima M, Costa MJ, Beltramini LM, Torres BB. Biochemistry and molecular biology education in Latin America and the Iberian Peninsula - Part 2. Biochem Mol Biol Educ. 2010; 38:63.

• Garcia CC, Freitas FP, Di Mascio P, Medeiros MH. Ultrasensitive simultaneous quantification of 1,N2-etheno-2'-deoxyguanosine and 1,N2-propano-2'-deoxyguanosine in DNA by an online liquid chromatography-electrospray tandem mass spectrometry assay. Chem Res Toxicol. 2010; 23:1245-55.

• Genaro-Mattos TC, Dalvi LT, Oliveira RG, Ginani JS, Hermes-Lima M. Reevaluation of the 2-deoxyribose assay for determination of free radical formation. Biochim Biophys Acta. 2009; 1790:1636-42.

• Gimenez M, Souza VC, Izumi C, Barbieri MR, Chammas R, Oba-Shinjo SM, Uno M, Marie SK, Rosa JC. Proteomic analysis of low- to high-grade astrocytomas reveals an alteration of the expression level of raf kinase inhibitor protein and nucleophosmin. Proteomics. 2010; 10:2812-21.

• Godoy LC, Moretti AI, Jurado MC, Oxer D, Janiszewski M, Ckless K, Velasco IT, Laurindo FR, Souza HP. Loss of CD40 endogenous S-nitrosylation during inflammatory response in endotoxemic mice and patients with sepsis. Shock. 2010; 33:626-33.

• Gorr TA, Wichmann D, Hu J, Hermes-Lima M, Welker AF, Terwilliger N, Wren JF, Viney M, Morris S, Nilsson GE, Deten A, Soliz J, Gassmann M. Hypoxia tolerance in animals: biology and application. Physiol Biochem Zool. 2010; 83:733-52.

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• Guaratini T, Cardozo KHM, Pinto E, Colepicolo P. Comparison of diode array and electrochemical detection in the C(30) reverse phase HPLC analysis of algae carotenoids. J Braz Chem Soc. 2009; 20:1609-1616.

• Guerreiro JR, Lameu C, Oliveira EF, Klitzke CF, Melo RL, Linares E, Augusto O, Fox JW, Lebrun I, Serrano SM, Camargo AC. Argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production. J Biol Chem. 2009; 284:20022-33.

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• Herrerias T, Oliveira AA, Belem ML, Oliveira BH, Carnieri EGS, Cadena SMSC, Noleto GR, Martinez GR, Oliveira MBM, Rocha MEM. Effects of natural flavones on membrane properties and citotoxicity of HeLa cells. | Efeitos de flavonas naturais em propriedades de membranas e em citotoxicidade de células HeLa. Rev Bras Farmacogn. 2010; 20:403-408.

• Horta BB, Oliveira MA, Discola KF, Cussiol JR, Netto LE. Structural and biochemical characterization of peroxiredoxin Q beta from Xylella fastidiosa: catalytic mechanism and high reactivity. J Biol Chem. 2010; 285:16051-65.

• Hugo M, Turell L, Manta B, Botti H, Monteiro G, Netto LE, Alvarez B, Radi R, Trujillo M. Thiol and sulfenic acid oxidation of AhpE, the one-cysteine peroxiredoxin from Mycobacterium tuberculosis: kinetics, acidity constants, and conformational dynamics. Biochemistry. 2009; 48:9416-26.

• Ishiki HM, Amaral AT-do. Three-Dimensional Quantitative Structure-Activity Relationship Study of Antitumor 2-Formylpyridine Thiosemicarbazones Derivatives as Inhibitors of Ribonucleotide Reductase. QSAR Comb Sci. 2009; 28:1334-45.

• Izumi CMS, Brito HF, Ferreira AMdaC, Constantino VRL, Temperini MLA. Spectroscopic investigation of the interactions between emeraldine base polyaniline and Eu(III) ions. Synth Met. 2009; 159:377-384.

• Izumi CMS, Ferreira DC, Ferreira AMdaC, Temperini MLA. The role of oxygen in the interaction of emeraldine base polyaniline with Cu(II) or Fe(III) ions in NMP solution. Synth Met. 2009;159:1165-1173.

• Jordão AK, Afonso PP, Ferreira VF, de Souza MC, Almeida MC, Beltrame CO, Paiva DP, Wardell SM, Wardell JL, Tiekink ER, Damaso CR, Cunha AC. Antiviral evaluation of N-amino-1,2,3-triazoles against Cantagalo virus replication in cell culture. Eur J Med Chem. 2009; 44:3777-83.

• Jordão AK, Ferreira VF, Lima ES, de Souza MC, Carlos EC, Castro HC, Geraldo RB, Rodrigues CR, Almeida MC, Cunha AC. Synthesis, antiplatelet and in silico evaluations of novel N-substituted-phenylamino-5-methyl-1H-1,2,3-triazole-4-carbohydrazides. Bioorg Med Chem. 2009; 17:3713-9.

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• Kirkali G, de Souza-Pinto NC, Jaruga P, Bohr VA, Dizdaroglu M. Accumulation of (5'S)-8,5'-cyclo-2'-deoxyadenosine in organs of Cockayne syndrome complementation group B gene knockout mice. DNA Repair (Amst). 2009; 8:274-8.

• Kowaltowski AJ, de Souza-Pinto NC, Castilho RF, Vercesi AE. Mitochondria and reactive oxygen species. Free Radic Biol Med. 2009; 47:333-43.

• Lall AB, Cronin TW, Carvalho AA, de Souza JM, Barros MP, Stevani CV, Bechara EJ, Ventura DF, Viviani VR, Hill AA. Vision in click beetles (Coleoptera: Elateridae): pigments and spectral correspondence between visual sensitivity and species bioluminescence emission. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2010; 196:629-38.

• Lima LG, Chammas R, Monteiro RQ, Moreira ME, Barcinski MA. Tumor-derived microvesicles modulate the establishment of metastatic melanoma in a phosphatidylserine-dependent manner. Cancer Lett. 2009; 283:168-75.

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• Lourenço AL, Abreu PA, Leal B, da Silva Júnior EN, Pinto AV, Pinto MD, Souza AM, Novais JS, Paiva MB, Cabral LM, Rodrigues CR, Ferreira VF, Castro HC. Identification of nor-beta-lapachone derivatives as potential antibacterial compounds against Enterococcus faecalis clinical strain. Curr Microbiol. 2010. [Epub ahead of print].

• Malavasi N, Rodrigues D, Chammas R, Chura-Chambi R, Barbuto J, Balduino K, Nonogaki S, Morganti L. Continuous and high level in vivo delivery of endostatin from recombinant cells encapsulated in TheraCyte immunoisolation devices. Cell Transplant. 2010; 19:269-77.

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• Mansano FV, Kazaoka RM, Ronsein GE, Prado FM, Genaro-Mattos TC, Uemi M, Di Mascio P, Miyamoto S. Highly sensitive fluorescent method for the detection of cholesterol aldehydes formed by ozone and singlet molecular oxygen. Anal Chem. 2010; 82:6775-81.

• Marcon AE, Ferreira Dde M, de Moura Mde F, Campos TF, do Amaral VS, Agnez-Lima LF, Batistuzzo de Medeiros SR. Genotoxic analysis in aquatic environment under influence of cyanobacteria, metal and radioactivity. Chemosphere. 2010; 81:773-80.

• Massari J, Tokikawa R, Zanolli L, Tavares MF, Assunção NA, Bechara EJ. Acetyl Radical Production by the Methylglyoxal-Peroxynitrite System: A Possible Route for l-Lysine Acetylation. Chem Res Toxicol. 2010. [Epub ahead of print].

• Mataveli LR, de Jesus Antunes N, Brigagão MR, de Magalhães CS, Wisniewski C, Luccas PO. Evaluation of a simple and low cost potentiometric biosensor for pharmaceutical and in vivo adrenaline determination. Biosens Bioelectron. 2010; 26:798-802.

• Matias F, Bonatto D, Padilla G, Rodrigues MF, Henriques JA. Polyhydroxyalkanoates production by actinobacteria isolated from soil. Can J Microbiol. 2009; 55:790-800.

• Maynard S, de Souza-Pinto NC, Scheibye-Knudsen M, Bohr VA. Mitochondrial base excision repair assays. Methods. 2010; 51:416-25.

• Maynard S, Schurman SH, Harboe C, de Souza-Pinto NC, Bohr VA. Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis. 2009; 30:2-10.

• Medeiros MH. Exocyclic DNA Adducts as Biomarkers of Lipid Oxidation and Predictors of Disease. Challenges in Developing Sensitive and Specific Methods for Clinical Studies. Chem Res Toxicol. 2009; 22:419-425.

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• Medinas DB, Gozzo FC, Santos LF, Iglesias AH, Augusto O. A ditryptophan cross-link is responsible for the covalent dimerization of human superoxide dismutase 1 during its bicarbonate-dependent peroxidase activity. Free Radic Biol Med. 2010; 49:1046-53.

• Medinas DB, Toledo JC Jr, Cerchiaro G, do-Amaral AT, de-Rezende L, Malvezzi A, Augusto O. Peroxymonocarbonate and carbonate radical displace the hydroxyl-like oxidant in the Sod1 peroxidase activity under physiological conditions. Chem Res Toxicol. 2009; 22:639-48.

• Mendes CB, Laignier EP, Brigagão MRPL, Luccas PO, Tarley CRT. A simple turbidimetric flow injection system for saccharin determination in sweetener products. Chem Pap. 2010; 64:285-293.

• Mendez-Sanchez SC, Martinez GR, Romão S, Echevarria A, Silva EF, Rocha ME, Noleto GR, Carnieri EG, Cadena SM, de Oliveira MB. The inhibition of lipoperoxidation by mesoionic compound MI-D: a relationship with its uncoupling effect and scavenging activity. Chem Biol Interact. 2009; 179:125-30.

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• Oliveira FL, Chammas R, Ricon L, Fermino ML, Bernardes ES, Hsu DK, Liu FT, Borojevic R, El-Cheikh MC. Galectin-3 regulates peritoneal B1-cell differentiation into plasma cells. Glycobiology. 2009; 19:1248-58.

• Oliveira JM, Mesquita DM, Hermes-Lima M. What's on the news? The use of media texts in exams of clinical biochemistry for medical and nutrition students. Biochem Mol Biol Educ. 2010; 38:85-90.

• Oliveira MA, Discola KF, Alves SV, Medrano FJ, Guimarães BG, Netto LE. Insights into the Specificity of Thioredoxin Reductase-Thioredoxin Interactions. A Structural and Functional Investigation of the Yeast Thioredoxin System. Biochemistry. 2010; 49:3317-26.

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• Orr AL, Lohse LA, Drew KL, Hermes-Lima M. Physiological oxidative stress after arousal from hibernation in Arctic ground squirrel. Comp Biochem Physiol A Mol Integr Physiol. 2009;153:213-21.

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• Possamai FP, Avila S Jr, Budni P, Backes P, Parisotto EB, Rizelio VM, Torres MA, Colepicolo P, Wilhelm Filho D. Occupational airborne contamination in South Brazil: 2. Oxidative stress detected in the blood of workers of incineration of hospital residues. Ecotoxicology. 2009; 18:1158-64.

• Prado FM, Oliveira MC, Miyamoto S, Martinez GR, Medeiros MH, Ronsein GE, Di Mascio P. Thymine hydroperoxide as a potential source of singlet molecular oxygen in DNA. Free Radic Biol Med. 2009; 47:401-9.

• Prolla TA, Bohr VA, de Souza-Pinto NC. Mitochondria and aging. Mech Ageing Dev. 2010;131:449-50. • Pueyo MT, Bloch C Jr, Carmona-Ribeiro AM, di Mascio P. Lipopeptides produced by a soil Bacillus

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• Ribeiro CM, Budni P, Pedrosa RC, Farias MS, Parisotto EB, Dalmarco EM, Fröde TS, Oliveira-Silva D, Colepicolo P, Filho DW. Antioxidant therapy attenuates oxidative insult caused by benzonidazole in chronic Chagas' heart disease. Int J Cardiol. 2010; 145:27-33.

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• Silva-Júnior ENda, de Moura MABF; Pinto AV, Pinto MCFR, de Souza MCBV, Araújo AJ, Pessoa C, Costa-Lotufo LV, Montenegro RC; Moraes MO, Ferreira VF, Goulart MOF. Cytotoxic, trypanocidal activities and physicochemical parameters of nor-beta-lapachone-based 1,2,3-triazoles. J Braz Chem Soc. 2009; 20:635-643.

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• Vilela MC, Lima GK, Rodrigues DH, Lacerda-Queiroz N, Mansur DS, de Miranda AS, Rachid MA, Kroon EG, Vieira LQ, Campos MA, Teixeira MM, Teixeira AL. TNFR1 plays a critical role in the control of severe HSV-1 encephalitis. Neurosci Lett. 2010; 479:58-62.

• Wang Z, Rhee DB, Lu J, Bohr CT, Zhou F, Vallabhaneni H, de Souza-Pinto NC, Liu Y. Characterization of oxidative guanine damage and repair in mammalian telomeres. PLoS Genet. 2010; 6:e1000951.

• Wilhelm Filho D, Júnior SA, Possamai FP, Parisotto EB, Moratelli AM, Garlet TR, Inácio DB, Torres MA, Colepicolo P, Dal-Pizzol F. Antioxidant therapy attenuates oxidative stress in the blood of subjects exposed to occupational airborne contamination from coal mining extraction and incineration of hospital residues. Ecotoxicology. 2010; 19:1193-200.

• Wosniak J Jr, Santos CX, Kowaltowski AJ, Laurindo FR. Cross-talk between mitochondria and NADPH oxidase: effects of mild mitochondrial dysfunction on angiotensin II-mediated increase in Nox isoform expression and activity in vascular smooth muscle cells. Antioxid Redox Signal. 2009; 11:1265-78.

• Yamaguchi LF, Kato MJ, Di Mascio P. Biflavonoids from Araucaria angustifolia protect against DNA UV-induced damage. Phytochemistry. 2009; 70:615-20.

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Capítulos de livros

• Alves, D P; Zanfolin, M; Vinagre, CM; Vieira, L.Q. Gnotobiologia. In: Valderez Bastos Valero Lapchiik; Vania Gomes de Moura Mattaraia; Gui Mi Ko. (Org.). Cuidados e Manejo de Animais de Laboratório. 1 ed. São Paulo: Atheneu, 2010, p. 295-313.

• Augusto, O; Myiamoto, S. Oxigen Radical and Related Species. In: Principals of Free Radical Biomedicine. Pantapoulos K; Schipper H (Eds). Nova Science Publishers Inc, 2010, prelo.

• Cardoso, A R; Queliconi, B B; Kowaltowski, AJ. Mitochondrial reactive oxygen species in myocardial pre and post-conditioning. In: Heinrich Sauer, Ajay Shah, Francisco Laurindo. (Org.). Studies on Cardiovascular Disorders. New York. Human Press. 2010, p 109-123.

• Chammas, R; Silva, DCP; Wainstein, A J A; Abdallah, KA. Imunologia Clínica das Neoplasias. In: J.C. Voltarelli; E.A. Donadi; I.F. de Carvalho; L. K. Arruda; P. Louzada Jr.; W. Sarti. (Org.). Imunologia Clínica na Prática Médica. 1 ed. São Paulo: Atheneu, 2009, p. 447-460.

• de Oliveira, I M; Bonatto, D ; Henriques, João Antonio Pêgas. Nitroreductases: Enzymes with Environmental, Biotechnological and Clinical Importance. In: Antonio Méndez-Vilas. (Org.). Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Badajoz: FORMATEX RESEARCH CENTER, 2010.

• Fernandes, D; Bonatto, D; Laurindo, F RM. The evolving concept of oxidative stress. In: Humana Press. (Org.). Oxidative stress in clinical practice: Studies on Cardiovascular diseases. New York: Springer, 2009, p. 1-41

• Ferreira Cravo, M; Welker, A F; Hermes-Lima, M. The connection between oxidative stress and estivation in gastropods and anurans. In: Carvalho, J.E.; Navas, C.A. (Org.). Aestivation. : Springer-Verlag, 2010, v. 49, p. 47-61.

• Francisco, Guilherme; TortelliI JR, Tharcisio Citrangulo; Chammas, R. Etiopatogenia- Melanócito ao melanoma. In: Fraoncisco A. Belfort; Alberto J.A. Wainstein. (Org.). Melanoma: Diagnóstico e tratamento. 1a ed. Marilia: LeMar, 2010, p. 37-45.

• Lall, A B; Cronin, T W; Bechara, EJH; Costa, C; Viviani, V R. Visual ecology of bioluminescent beetles: visual spectra mechanisms and the colors of optical signaling in Coleoptera, Elateroidea: Lampyridae, Elateridae and Phengodidae.. In Bioluminescence in Focus: A Collection of Illuminating Essays.. Finlândia: Victor Benno Meyer-Rochow, 2009, prelo.

• Laurindo, FRM. Estresse Oxidativo Vascular. Bases Moleculares das Doenças Cardiovasculares. São Paulo: Atheneu, 2008, p. 433-443.

• Miyamoto S, Arai H, Terao J. In: Aldini G, Yeum KJ, Niki E, Russell RM (Org.). Enzymatic Antioxidant Defenses. Biomarkers for Antioxidant Defense and Oxidative Damage, Hoboken: Wiley-Blackwell. 2010, p. 21-33.

• Miyamoto, S; Almeida, EA; Nogueira, L; Medeiros, M H G; Di Mascio, P. Evaluation of malonaldehyde levels. In: Tania Zenteno-Savin; José Pablo Vázquez-Medina; Doris Abele. (Org.). Oxidative Stress in Aquatic Ecosystems. Hoboken: Wiley-Blackwell, 2010.

• Nagai, MA; Chammas, R; Bretani, MM. Oncogênese e Fisiopatologia do Câncer. In: M.A. Martins; F.J. Carrilho; V.A.F. Alves; E.A. Castilho; G.G. Cerri; C.L. Wen. (Org.). Clínica Médica. 1 ed. São Paulo: Manole, 2009, v. 3, p. 448-466.

• Rocha D R; Ferreira, V F; Araujo K G L; Santos W C. ADVANCES IN DRUG DISCOVERY TO ASSESS CHOLINERGIC NEUROTRANSMISSION. In: P. Mossillo; J. Pinzini. (Org.). Biopharmaceutics and Drug Hypersensitivity. NY: Nova Science Publishers, Inc, 2009, v. 1.

Livros

• Sauer H, Shah AM, Laurindo FRM (Editors). Studies on Cardiovascular Disorders, Oxidative Stress in Applied Basic Research and Clinical Practise. New York. Human Press. Springer. 2010.