KKU. Vet. J. Vol. g No. 2 JULY - DECEMBER leee

tJvt:{lp{?1iil{Ufifi Basal Cell Carcinoma

dfi:{e,ierqrravq nirlugrTt : :'lu tlu#sri{hs

Pigmented Cystic Basal Cell Carcinoma in a Dogl A Case Report

Rizo: sl-ooisilu't' il::Krr't oi'ro{suur' a1:u11't: aJetfi[flc{ 'Sirikachorn Tangkawattana 1 Prasarn Tangkawattana 2 Arayaporn Macotpet 3

AJI

u?lfi9luo

ntrratyoetrfiorfiuoi':orlliro.tr14fi2ornriourfi'o.:on (tine needle aspiration, FNA)CJ

.J ^ v e a dv iuu unsrfiu oruil:rxJril 4 iJ tqi'lo.lrunrlnrju?TU5t't iloi'tulj1.l fl1€1010.1frUfl1 1Un 0d t{tl e

ufioq fi{tfir1nnrrnr'ra?ffo#rLvrr.rirynii'ivrErrudoroiu'jrrflu,drrrn,u*rTulr (melanoma), 4^

riqiqrnnt:e:rqluodunr.:anwurSlrrrjre.Lnlnr:toaJgllteu Fontana-Masson techniqueo q I /,

I

rrLun:ru':rqur.rorfiriJutrrT.:rryadrld'n (basal cell carcinoma) tflofrfrnr:a:n'ruI

:.:eriqnrsJnrfiu rrn:finlrirorinruturiaurfi'0.:an (pigmented cystic basal cell carcinoma)qq$

fl r r eL uer n si l.r r o.r r ^

n., r r rr u{r a o r i fivirlfivr :r u'i r qr*r o d r J fi r fi u ru rI e ui 6 rnrn fuv,^X ziq ^ ;d < v a "6rv ffUtfj n.ll:n['trjnruaruoLuu5trruvtrutqnnlrt:.:ootJ'l9t'lu 0.1?111fin'l:?l.tQ; s

AbstractSerosanguineous fluid obtained by fine needle aspiration (frun) from a tumor mass

on the neck of an Alsatian, female 4-year-old dog was cytologically diagnosed as mela-

noma. However, the tumor was histopathologically examined by Fontana-Masson staining

technique as pigmented cystic basal cell carcinoma which bore melanin pigment and cysts

inside. The different result from the two methods indicated that the sample obtained by

FNA did not contain the cancer parenchyma leading to the incomplete diagnosis.

cirdra-q,; : r:u5.:firuril t"rn{trry-n :.:nirn nrtir

Keyword : skin cancer, basal cell, pigment. cyst

' ntoiTrnrr?6rivrrr fl rutflg? ttv,ryruff'rfiqr: tJu't?mu1d url o14ttnt[

Department o{ Veterinary Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University

' nrnitrnruinrnqrsqi nrx:#qr$rryruaraqi iluriilf.Jrn-urtouttriu

Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Khon Kaen University

' I:.:il urur n ao{ qr rw o-qr uilyr usr as}i rivrin ur #lt auttriu

Veterinary Hospital, Faculty of Veterinary Medicine, Khon Kaen University

JJ

34 xl:#axuilr4uct16{m{ ars. iJfi g arirfi 2 nrnglrlal - i'narsrel 2542

o

lJ11l.t1

rurr5.:fi':rari.:tfie basal cell

carcinoma (Bcc) fifiovilovjr basal celldt a Aa

epithelioma un; basaloma til1{tJYtr.lYltJuvl616'

o?1eJ5lULtr{ lilil1niln [ueJul+ul^lu?''l

n1r#il zu*fi L[fl.] fn q:r'[rT o r fi q riJur; u v

t'r n1u'r uflr rur:n n:v oi'u lv{rfi q il v r5.:tfi el

Xu - ,6t u dY *vu [o rtor luflo?l{uu.] L]JYI:-t1Jfl'tt149tL!l,[To

(Murphy and Elder, 1991) vrtrdfintfio3q-Aauue1lULUUirrr[zu?141.[.]reJ1u0% tqttn il? o0

tyeiruon unrt'ru{1 (aaxer and Thomsett,

1990; Gross et al., 1992) loufiorifid,au6tY'n1:ni[urrrJlfr{ni1[ugul Luttruruuut:lrrq9

rir.:orqrafi u 7 -10 iJ riullu'[oi'trn (crossq

. ,61 v v \A td vet al., 1 992) ttqrLl.[fll,[flu.] L]JtJlJ0eJdttl{lto

rtrad'fl o.i BCC rfioolnLmnd'1fi ouI

pluripotential fi o-nurxlnd'rutr

(basal cell ) fi turo ufi n rrnr a ritud'u

rJ:vifi#sridjasdu a d

flulj1{U5'0 n ttLailutllflt3.JU 0'l E{qq

rj:senna + iJ rd'rtor#rtnqrfiuri'ourfioia .l ,J

tonfi fiture"lfi qjfluilrriaI 1 ruril:rlrrxd' 'ci a I A

1 il 0 uyllrrt? il9.1?14u.]fl?iln1n01'l{fl ?''lllql

etdlu',rqrdlrr.i'rguunnrt:J:3il']il 4 flel. nlT

(U

to'rverq tfi uei'? o drtoi'r uifrnlT fine needleqJ

u X i.q.aspiration a'lnnoutu0.l0nuvl-l IH1lu

'[qi"jr fi t o r rH nr fr'n :*ruvlarJ urfi o q (serosan -guineous fluid) orin"ru'[u t{nnlTar?o

vd^&vtrr uq au ilr.l tfr n n? r u1 t:J 0.1 9l1,lylu?''l]J T3n 0 u

qi'r u rfi e td o q Lter{ tLa r Lil nrlu'lrqi' ( melano -. iacyte) frfrltn:uo (granule) ddrqrnrdil

d' " A 06l e , <r Xtil1.{o'ru?ue.Jln Tt.lTl'l LnnnTiu?'lt:Jutu0

.lOnTfiq well-differentiated melanomadaneo s t

n't u fi d.1 d9t't ttl l ?1 u Lo ?.l1 n1TTn H'l Lq u n''lruXY

fl R Un:TSJqqfl0uLlio.l 8n 0 anr.1fi 3.Jo tLntMvr u XLo fl.i n 0ut1.[0 3J1q:?o'] uaauil'1.:0 dl lu']5'

a,Mr111il10A L!

A.' AAa nu 6uu 1l-lil ?\ u 1 fi ?11 u1

v

rlourfioron?ure 4x5x3 ril. Ieu

il:rrurrx nnrJnn o r oi': u fi lari.rdr fi rrrunflq

, A o 6l e d Vl e' u Xil n il o u qT.] n nl.l s.lYl'l [14 t141-[ [9lxln al,ttua

qqlX' v

,:anfr:Jrrnouqirl4riol (lobutateo mass)U

A ,t u a Y Aa u at'.: :l r.: lnt tt fl rl 0 0 n t 9.1 u 114 t 14 u n.l u'l ?'l 3.l zu 1.[.] fl

qlq

rirornoir (:rJfi 1 n) rfioriletirrdrliltu\a* X a d vl eu r I eriouui+or o n0{ t141.{ lglto taux']lJTYn 0u ox EJ

X j , j<, A^, Ztua tu o 6ixuYltil1+1lu 0 u ttnvfl'luvlt:lun{u'l{qoY o a 6l r 4aurornq.l 11n3il10.1t14R',] [flu1-[[fl 091

(serosanguineous fluid) l::oori

tJ?T1+9t

nntttrn&-oeL

stratum basale 1J 0{5u141.[.: nlilT'lT1 lJ11.]

f e j j^ ,L a u ravLnT{ &T'1.1 01.t?19ro 0 Unil il?llu{ Lt$il0nl*{uvqY ^ r vl rdo.tt9ilJ (primitive) n11 !!0r [ilil desmosome

(Murphy and Elder,1991;Gross et al.,.:vu1992) uonornfrursrlrtnilLnnt*iuy

46uqfi (secondary feature) d.:'[qirrri nr:

flrfliltetnlfiil (melanization) ttalnlrtficlZ . oav d i

ft.lll't (cvstification ) nf LuarrulTntTUntoq\

o'rtnrrjr pigmented cystic basal cell

carcinoma (eCnCC) drriJuruvrT.:rfiqfiq uMd/l r r

1\U 11.[fl1.[1 [9r LllU0UUn

KKU. Vet. J. Vol. e No. 2 JULY - DECEMBER leee 35

j^! il I Al:'l a ?1'l.l Q n y'l u'l 5'l ?1 u'l 1 l u ?'l

o X N, e , d vn 0uLuauil:3n 0Lo'l unaSJ tsnn tLsfl Lldl

< T M d cr d v , ,ArsRrirerar[u'tsqirfJurrynciriai'n (;iln 1 1)

uein! rqn frr nrlur lrori (melanophage)rVl es d t

ttn:n0u tolj'l.l tunnt:JTf, 0uT'lSJnu0utuu{a{

,;oXu,nalJ 61 nT;alu?1?n816tua tXJllL desmo-

9r

some oLi:ru'jr.riryoffiGoriu v{L atypical{d ^ ivr -- vt

mitotic figure lJO.Jtsndufiefi"LoYtrr'til

rfl fl dr r n rluls qi-riJ u ts n dfi fi *n :un fiqJ

Y-,6ldd5lll-t''t $t-r d tlj eJ u T:a o u n 1 u Lutg n n ;JlJ 1-['1 q Lfi ru

& , <, ^.frun'jrrqndrJnG (rrypertrophic melanocyte)

,Tuzwu n:ral u 0 il oreL lgr:.: o5r.:errau ( stroma)

il9

ttRrrty'r:nturru'i1{n6isJrqnd'ru:uT.: (:rl9 'qJ

j j . u X A - deYr 1 n ) r3.J0il1n1:u0lJt1l,0tu0o'lufi'u03Jv'ltfl14

Fontana-Masson technique dlrilufifitflue v a o6l e X

nlTg A il:.t ft'l q n til fi']141-[ Y]'l t14 fi''l3JlTnL.]Tq

qfr rru r.i.r r o.: t il n 1 fi ufi o ritu url u.t I E.:{rsn drl nrTulrJei-fi rin:n'hJ o $lueiruoi'x 1su q , ,A 6A'tciorrrrorau (::JYt 1 .t ) tsn8il0ufl03J\qt / \

ro:rni;r ri.r rfl urT n {tfi o 16 lr ffr-lfi r rllu,i*2qfA

frx.u01{ t lJ0.ln0%tua.l0n [uL1{1^lu?lv,{11

^ X .i vlenlTtnotu0LU0nrs0n Log

in :gol:rrrw rrr?i r lrl o.: I : ri.l prqmented cystrc basal cell carcinomaa"

( n ) nh ururr or r: u5i ifi o o:roe-r ue']rrl 6ir ilL aidrfr fi frrir qr noir

(l) nnrulo.:rrnairrri.: (" ) n::orrorjvj'lt:.J wrmdarra{:.rflienuilnrfiuRrirorneirotluler:.:airl"*,.r* uot

rur:nlunc!rr o.l rqn d':Jvt5r (H&E, x40 )* z;(e) ruf-lrorn.:urfrl:rnousi'rutsad-rurril (+>) (H&E, x4oo)

( r ) u,rn d.rll nrlu'lsyi I ' ) fifir,:e ie n rrnrflur:ro a rjratrnr rluoirtrynd-un:rttu.: urynd ( Fontana - Masson. X40o )

r1li4 1

36 ?1:#orxuflvrucl'r6{a{ ilfl. flfi 9 ilu-ufi 2 nrngraal - #ltmnru 2542

Ad?o1:€u9{a

ilvr5{tfiq PCBCC finnrcilrfieror

rff uqr r:J eir n d'r u n 6 r rYu rfi'r r t n rfi er

me\anoma r""ru tou:ou\:qt\NGuuuo:fifi

rdrur o.r:.: oiorqfi er eer o {nr utu ( eulley9

and Stannard, 1990; Bigler et al., 1 996)A - , j, < A ^o - (fr.:ntnqr 0 u-t.t?tfl{ tn:J t1 t 0't140ou?l1.: tsnil

i n ur'[ri fi n si l tmn d'il v r5.r fi o ruT qit u rrd"tc

ors a vyirtffi fio d'u'[ri sr ri : niTe u:ru'ir ril u

rfiu.:rfio melanoma d.:rarnvfrnr:tffuxi,

n'l a ul.l tl,t0 !u a fl.:qT10?11{aRy{ U'lT'lYlu'lA-dt

tl^t 0 qrxo 0 n uilt t6lta n Ltfl v:u tLlJ L n1T0 91 0l')

1r o.r Ls CI,i'rfl o r o n o ; vfrtri'nr :i fi o Ei'u'[qi

6 a X ,q dRilurni u0ntnu00lnuLLn1 Lun1Tflnl9'lql

vexnt{a nil il"]5.?Ylu'l u.lq a{ ttun?u0aunu iuo

, A aa , A ?.{ Anlj A.l g 0 ulj14U.:3J nnUilYFlfl1Unn.ln}t

IA

fl'r 14 TL il T8J'l [U tt n 3 nl Tn T3 0'l u q ?1 0 i T.] 9l

& a dv't o n t il d't f.i141.[1.i 3J Fl ?'l 3J fl N 1 l 1A5n U ? U'l q Lt n i

n1Trrq n u1J1a.tlJ o.J rsn d'rilorluttqilo u qr.l

,51riruriavir.:n1 u Lun.:d.l tiiurunrurorn n1T

q

Ae,Ajylttanu'l.lnniltfl0lJttnrqlun.l tel0ilnqqJ

rir,i'e o a n'[:J vr:rlri'rra fi o dufi'ir.r rfi odu

(Gross et al., 1992)A 9tfr I rrri'jtnn:g61.[3 mitotic figure qnlf

dt-,XaifJuo"::-i.18ff.: nrlr ruir I tt:.t (malignancy)

odrnafi.tra{sJtt5.t rrqiafT raiu PCBCC rr#r

ffn:grxvnrtQ0l r ulGiil urdu 1 I o.: ifi'o.: anq

aeXu.l fl'l tJ'l Til 1l.l u 0 n il t o ?'l ul'l u tt:.]lj 0.1 tu 0 { a n

'[qi'6noiru Ieuurnrflu:-J:vtnn solid typectoiavt^i

0 r t]J 1.[T1-[9r yl lJ o']'t 3J T'l fJ ttT{ il1 n n?l ruo 0 u 6l

(Nielsen andCole, t gOO htlaa Putley and

Stannard, 1 990) rrqiarjrr\:fiErrru \rifi

T'r u.r'run1Tuilinr;01 ulj 0.1 PCBCC lu#qr{u,<*Xq,3aA

u n v Ln v,'t :-.t tfi 0 n il r tr.l 1l Llaqt 0 3J 1.['l Lu d a { r 0 u9l

vAvdlj't.t tfi u { n 0 ueJ 3 [T.1 ttgl fl] ll1 Tn n L nl : ln 9l

dulrail (recurrence) '[oi'rir.: (Baker and

Thomsett, 1990; Gross et al., 1992)- Xa t 'A vt d

<,[r u tfi q p] ntisi'1 01q o : n n-t? Lo ?'l tl v tr.l 51loq

d'[ri fi n'lr ru ir u ur.] un s fi1 ilr :n 6'e:r eJ o Litu

nairurfi'a.:onrfiq basal cell tumor (Pulley and

Stannard, 1 990) fi'[d' et-{#unr:fn]erd^XuVlc/Tad

eir t5.t ruo iiot rut:n :n ul [9] [9] u ? 5vl'.l.]

- YX r a , J-, dfl 0 u n rTtJ Yl.l u0 g 91 0.1 9l 9l t 0'l fl ? ul'l tlj u3J 3 tr.l

dl e A q - ^ & vudo o n LliulJ 9l nnt oil o.: nunlTLnoljl,[lJ'l Lq 0 n

d:ilq

tdo{ernilr r5{tfiq pigmented cys -aa i

tic basal cell carcinoma ilfln:*iugileJ0.ld V ev t t v A v X

tnu Lo o1 u qt t! n1fl a1 u gt fl .l nu tu0.l 0n

-Ydumelanoma il1n g{uunlrtnilq?0il'l.l, i q4,-

a.t n:lo tlt0?1''lnlT?uaaunl{ tryn 0??'lu1

lquifi fine needle aspiration oroolvirtri'vto YVr+4

r ua a u i;'i 9r il n 1 q L o u 1 n q ? 0 u

-.l.1 1.11{ L 3J AJ L U 0

ia6:d^eqar0afi5otfinnljo{ilvtltqlotl'l9l'lu Lunriu,Xue

tT l.tlAn l T? 1.i0 il U Yl'1.: o'1 1-10 CI 1 l u-l 5x 11 u'l :? ilu v aa q e -dnunt: u a ru airt isl:90r Lu zu n n1:9lr?0 il n n

qJ

vla0,:il1nn?']

KKU. Vet. J. Vol. g No. 2 JULY - DECEMBER leee

AAInflnn::illJ:vfllfllJol1oufitu Associate Professor Hiroyuki Taniyama, Department of Veterinary

Pathology, School of Veterinary Medicine, Rakuno Gakuen University rl:rtilei ,, q u a a d s dq dfi rl 1rLunr:i ffa al iluuu uay fl {ufr il ut nln:ri i ttasfl t*qro't 2T U'l tlJ D'l tTa u Lunl:taru ilti q I 9

u <& aR IROtX{FtLil0

Baker, K.P. and Thomsett, L.R. 1990. Canine and Feline Dermatology. Blackwell Scientific

Publications, Oxford. pp.1 7 2-203.

Bigler, C., Feldman, J., Hall, E. and Padilla, R.S. 1996. Pigmented basal cell carcinoma in

hispanics. J. Am. Acad. Dermatol. 3A(2):751-752.

Gross, T.L., lhrke, P.J. and Walder, E.J. 1992. Veierinary Dermatopathology: A Macro-

scopic and Microscopic Evaluation of Canine and Feline Skin Diseases. Mosby

Yearbook, St.Louis. PP.320-350.

Maloney, M.E., Jones, D.B. and Sexton, M.F. 1992. Pigmented basal cell carcinoma:

investigation of 7O cases. J. Am. Acad. Dermalol.2T(1):74-18-

Murphy, G.F. and Elder, D.E. 1991. Non-melanocyticTumors of the Skin. Armed Forces

lnstitute of Pathology, Washington, D.C. pp.47-60.

Nielsen, S.W. and Cole, C.R. 1960. Cutaneous epithelial neoplasms of the dog: A report

ot 153 cases. Am. J. Vet. Res. 16:417-432-

Pulley, L.T. and Stannard, A.A. 1990. Tumors of the skin and soft tissues- ln: Tumors in

Domestic Animals, 3rd ed. J.E. Moulton (ed). University of California Press,

Berkeley. pp.23-87.

37

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