Preparo de biblioteca para sequenciamento Exemplos dos ... · DNA Insert Rd2 SP Index Rdl SP Index...

Introdução à Bioinformática – IQB001

Rafael Dias Mesquita rdmesquita@iq.ufrj.br

Laboratório de Bioinformática

Departamento de Bioquímica

Preparo de biblioteca para sequenciamento Exemplos dos kits TruSeq e Nextera da Illumina

Preliminar: PCR

http://bioinfo.iq.ufrj.br/graduacao/links/08%20PCR.swf

Kit TruSeq Illumina

DNA double strands created based on FW and RV strands

Strand specificity

U U U U U U This strand is NOT used as template on PCR

DNA double strand created based only on FW strand

Strand specificity

U U U U U U This strand is NOT used as template on PCR

DNA double strand created based only on FW strand

https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf

Kit Nextera Illumina

https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/nextera-dna-flex.html

Kit Nextera Illumina

https://www.youtube.com/watch?v=-kTcFZxP6kM

Resumo de vários kits de preparação da amostra