Upload
sunil-saldanha
View
202
Download
0
Embed Size (px)
Citation preview
CRYOBANKEMPROCELL CLINICAL RESEARCH PVT. LTD., MUMBAI
FETAL TISSUE EXTRACT
Liver
Brain
Spina
l cordKid
ney
Thymus Lun
g
All tis
sue Heat
Pancre
as
Cord
tissue
Placen
ta
Skin +
ribs +
musc
le
Head h
air sk
in
Bladd
er
Body
skin
Cartil
ago
Trache
o-bron
chilla
ris com
pleks
Abdom
en sk
in + m
uscle
Stomac
Bone
Splee
n Eye
Limb m
uscle
Face s
kin
Esopha
gus
Thyroi
d glan
d Gut
Suprar
enal g
land
Aorta
834
490
287
422
150
827
377409
82
431
523 × 10
104 × 10
442
71
231
138
30 36
162
12
261225
439
266
3662
448
171127
Amount (vials - 1.8 ml): 14444 vials ~ 26 l
FETAL ORGANSPECIFIC PROGENITOR CELLS
Liver
Brain
Spina
l cord
Kidne
y
Thymus Lun
g
All tis
sue Heat
Pancre
as
Cord
tissue
Placen
ta
Skin +
ribs +
musc
le
Head h
air sk
in
Body
skin
Abdo
men ski
n + m
uscle
Stomac
Bone
Splee
n Eye
Limb m
uscle
Face s
kin Gut
Suprar
enal g
land
Aorta
337 × 10
423 × 10
262
702
204
466
720
335
187 178
523 104 442 41
77
6
6124
135
4912
64 4717
Amount (vials - 1.8 ml): 11276 vials ~ 20 l
EmProCell Laboratory StructureEmProCell
Biotechnology Lab
Bio-Safety Lab
R&D Lab
Sample Processing
Manufacturing
Cryobank
ELISA
Cell Culture
Pathogen Detection
Karyotyping & Hybridization
Sterilization lab
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd. 14/10/2012
Donor Screening
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd 14/10/2012
Pregnant women blood ELISA test for:
HBV
HCV
CMV
HTLV
VDRL
Chromogenic End-Point Assay (QCL 1000, Lonza).Kit Sensitivity: 0.01 EU/ml
ELISA Lab
Endotoxin Detection
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd. 14/10/2012
Pathogen Detection Lab
Standard kits used for :
• DNA Isolation
• RNA Isolation
Nucleic Acid Isolation
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd 14/10/2012
HBVHCV CMVEBVHSV1 &2HIV 1 & 2
Treponema pallidum
Mycoplasma hominisUreaplasma sp.Chlamydia all speciesToxoplasma gondii
Pathogen Detected
Pathogen Detection Lab
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd.
Pathogen detection Lab
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd. 14/10/2012
Cell Culture lab
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd. 14/10/2012
Sterility testing of samples
Outsourced to : Super
Religare LaboratoriesTest performed : Culturing of
Aerobic bacteria Anaerobic
bacteria Yeast/ molds
Method Used : Chemi-luminoscent Assay
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd. 14/10/2012
Sterility testing of samples at EmProCell
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd. 14/10/2012
Karyotyping Lab
Traditional karyotypingTo check on any genetic abnormalities in samples
Only Sample with normal karyotype selected
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd. 14/10/2012
PASSPORT OF CELL SUSPENSIONSample ID: (_ _._ _._ _/_)(_)(_ _ _)(_ _ _ _ _ _)
Type of testing Marker Result Marker Result
Screening Donors(ELISA)
HIV 1 [ ] CMV [ ]
HIV 2 [ ] HTLV [ ]
HBV [ ] VDRL [ ]
HCV [ ]
Cell test-control(PCR)
Pathogen Result Pathogen Result
HBV [ ] HCV [ ]
CMV [ ] EBV [ ]
HSV 1 & 2 [ ] HIV 1 & 2 [ ]
Treponema pallidum [ ] Mycoplasma hominis [ ]
Ureaplasma sp. [ ] Chlamydia (all species) [ ]
Toxoplasma gondii [ ] X-Y chromosome detection [ ]
Microbiology controlSterility testing
Pathogen Result Pathogen Result
Aerobic m/o [ ] Fungus [ ]
Anaerobic m/o [ ]
PASSPORT OF CELL SUSPENSIONGestation period of the
human fetus
(mark as [√])
Weeks Result Weeks Result Weeks Result
6-7 weeks [ ] 13 weeks [ ] 17 weeks [ ]
8-9 weeks [ ] 14 weeks [ ] 18 weeks [ ]
10-11 weeks [ ] 15 weeks [ ] 19 weeks [ ]
12 weeks [ ] 16 weeks [ ] 20 weeks [ ]
Cell type
(mark as [√])
Hematopoietic fetal liver stem cells [ ]
Fetal liver hepatoblasts and hepatocytes [ ]
Neural progenitor fetal brain cells [ ]
Quantity & Quality Total Cell Amount per 1 ml
Vible-Cell Amount per 1 ml
Amount of CFU per 1 ml
Amount of mononuclear cells
Karyotyping (FISH)
Transplantation unit: amount of CFU/amount of viable cells
Cryo-protector (mark as
[√])
DMSO [ ]
PVG [ ]
Any other (Name)
PASSPORT OF CELL SUSPENSIONPhenotype of fetal liver hematopoietic stem cells
HLA typing (Type)
CD Marker(mark as [√])
CD Marker Result CD Marker Result CD Marker Result
CD4 [ ] CD56 [ ] CD133 [ ]
CD8 [ ] CD62E [ ] CD135 [ ]
CD12w [ ] CD62P [ ] CD141 [ ]
CD19 [ ] CD69 [ ] CD144 [ ]
CD24 [ ] CD74 [ ] CD158 [ ]
CD27 [ ] CD83 [ ] CD163 [ ]
CD31 [ ] CD90 [ ] CD184 [ ]
CD34 [ ] CD100 [ ] CD202a [ ]
CD42 [ ] CD105 [ ] CD326 [ ]
CD45 [ ] CD117 [ ] CD133 [ ]
Marker Phenotype of fetal liver hepatoblasts and hepatocytes
Result Marker Result Marker Result
AFP [ ] HLA-A [ ] CD74 [ ]
CD326 [ ] HLA-B [ ] CD135 [ ]
Albumin [ ] HLA-DR [ ] CD326 [ ]
C017-1A antigen [ ] CD68 [ ]
PASSPORT OF CELL SUSPENSIONPhenotype of neural fetal brain progenitor cells
Marker Result Marker Result Marker Result
Nestin [ ] HLA-A [ ] CD133 [ ]
β-tubulin III [ ] HLA-B [ ] CD271 [ ]
Vimentin [ ] HLA-DR [ ] CD304 [ ]
GFAP [ ] CD24 [ ] CD326 [ ]
NCAM [ ] CD25 [ ] CD69 [ ]
GalC [ ] CD74 [ ]
Genetic abnormality in cells if any detected by karyotyping:Please enlist __________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________
THANK YOU
Bio-Safety Dept- EmProCell Clinical Research Pvt. Ltd. 14/10/2012